Dual Effect of Protein Kinase C on the Induction of DNA Synthesis by Colcemid in G0-Arrested Human Diploid Fibroblasts

Cell Structure and Function

Toshinori Ide¹  Kazue Tsuji¹  Akemi Ueno¹ 

[1] Department of Cellular and Molecular Biology, Hiroshima University School of Medicine

关键词: TPA;    protein kinase C;    antiproliferative action;    human diploid fibroblasts;   

DOI  :  10.1247/csf.16.73

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(26)Cited-By(3)G0-arrested human diploid fibroblasts, TIG-1, was stimulated to induce DNA synthesis by serum, epidermal growth factor (EGF), colchicine, colcemid, or 12-O-tetradecanoylphorbol-13-acetate (TPA). The induction of DNA synthesis was mediated by protein kinase C (PKC) when stimulated with TPA but not when stimulated with other agents. When TPA-stimulated cells were immediately treated with colcemid, induction of DNA synthesis was reduced. This reduction diminished when colcemid was added more than 6 h after TPA treatment. Conversely, when colcemid-stimulated cells were treated with TPA, induction of DNA synthesis was also reduced. This reduction was enhanced when the interval between the addition of two stimulants was extended. PKC-deprivation abolished both stimulatory and inhibitory effects of TPA on DNA synthesis. Staurosporine blocked an induction of DNA synthesis by TPA but appeared to be ineffective on the inhibitory action of TPA on DNA synthesis by colcemid. These results suggest that the inhibitory effect of TPA on the induction of DNA synthesis by colcemid is mediated by down regulation-sensitive and staurosporine-insensitive PKC.

【 授权许可】

Unknown