Microwave-Assisted Lipase Covalent Immobilization and Improved Its Thermal Stability Using Alcohols to Quench Excessive Activated Groups on Support Surface

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Cheng Zhou¹  Shubao Shen¹  Anming Wang¹  Shemin Zhu²  Zhiqiang Du¹ 

[1] College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology;College of Materials Science and Engineering, Nanjing University of Technology

关键词: Immobilization;    Microwave Irradiation;    Quench;    Blockage;    Mesocellular Siliceous Foams;   

DOI  :  10.1252/jcej.08we304

来源: Maruzen Company Ltd

【 摘 要 】

References(28)Cited-By(3)Microwave irradiation technology is used to covalently immobilize lipase at low temperature to improve enzyme properties. The specific activity of lipase immobilized under microwave irradiation was 114.4 U·mg–1, 1.23 times of that for a traditional method without microwave irradiation. The Km and Kcat/Km of microwave immobilized lipase was 0.12 and 1.26 folds that of free lipase. Moreover, alcohols were used to quench the excessive activated groups on MCFs surface to improve thermal stability of immobilized lipase. Residual activity of immobilized lipase quenched by ethanol, retained 60.64% after incubation at 50°C for 5 h, 2.36 and 1.99 times higher than those of free lipase and immobilized lipase which was not quenched by alcohols, respectively. The results showed that microwave irradiation could improve lipase covalent immobilization and quenching remaining groups could enhance thermal stability of immobilized lipase.

【 授权许可】

Unknown