JOURNAL OF CHEMICAL ENGINEERING OF JAPAN | |
Effect of Electrostatic Interaction on Reverse Micellar Extraction of Large Molecular Weight Proteins | |
Yoshinobu Kawano2  Isao Komasawa1  Koichiro Shiomori2  Ryoichi Kuboi1  | |
[1] Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University;Department of Applied Chemistry, Miyazaki University | |
关键词: Reverse Micelles; Extraction; Protein; Electrostatic Interaction; AOT; | |
DOI : 10.1252/jcej.33.800 | |
来源: Maruzen Company Ltd | |
【 摘 要 】
References(10)Cited-By(10)Reverse micellar extraction of various proteins of large molecular weight and oligomeric proteins, as well as small ones, was carried out using conventional AOT reverse micellar extraction systems at various pH values. The conformational change of the proteins by electrostatic interaction with AOT was also measured using a CD spectrum. Extraction behavior and conformational change of the proteins were discussed from their electric charge properties and electrostatic interaction between protein and AOT. Most of the proteins were effectively extracted into the reverse micellar phase at around zero surface net charge. Formation of aggregates and denaturation of proteins were observed in the region of positive net charge higher than 10.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912080694742ZK.pdf | 19KB | download |