A Phase-Segregated Model for Plant Cell Culture: The Effect of Cell Volume Fraction

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Wei Zhang²  Shintaro Furusaki¹  Anton P. J. Middelberg² 

[1] Department of Chem. and Biotech., Graduate School of Engineering, The University of Tokyo;Department of Chemical Engineering, The University of Adelaide

关键词: Plant Cell Culture;    Phase-Segregated;    Modeling;    Cell Volume Fraction (CVF);    Structured Model;   

DOI  :  10.1252/jcej.31.469

来源: Maruzen Company Ltd

【 摘 要 】

References(12)Cited-By(1)Plant cells are characterized by low water content, so the fraction of cell volume (volume fraction) in a vessel is large compared with other cell systems, even if the cell concentrations are the same. Therefore, concentration of plant cells should preferably be expressed by the liquid volume basis rather than by the total vessel volume basis. In this paper, a new model is proposed to analyze behavior of a plant cell culture by dividing the cell suspension into the biotic- and abiotic-phases. Using this model, we analyzed the cell-growth and the alkaloid production by Catharanthus roseus. Large errors in the simulated results were observed if the phase-segregation was not considered.

【 授权许可】

Unknown