Culture of Red Beet Hairy Root in Bioreactor and Recovery of Pigment Released from the Cells by Repeated Treatment of Oxygen Starvation

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Setsuji Tone¹  Yasuhiro Hongo¹  Masahiro Kino-oka¹  Masahito Taya¹ 

[1] Department of Chemical Engineering, Faculty of Engineering Science, Osaka University

关键词: Biochemical Engineering;    Plant Cell Culture;    Hairy Root;    Red Beet;    Pigment Production;   

DOI  :  10.1252/jcej.25.490

来源: Maruzen Company Ltd

【 摘 要 】

References(18)Cited-By(15)In the culture of red beet hairy root, the red pigment (mainly betanin) was released from the cells into medium when the cells were subjected to the culture condition under O2 starvation by keeping the concentration of dissolved oxygen in medium at 0 ppm. The amount of released pigment increased with increasing time length of O2 starvation during 26 h. However, an O2 starvation time of less than 16 h was employed to shorten the lag time for cell growth resumption after this starvation treatment. An adsorption column with a hydrophobic resin, Sepabeads SP 207 (styrene-divinylbenzene copolymer), was incorporated in a bioreactor system and the long-term culture of hairy root was carried out with repeated operations of cell growth, pigment release and recovery. In the culture with an O2 starvation time of 16 h, an extracellular production rate of pigment of 11.3 × 10–5 kg/(m3-broth·h) was obtained on the average.

【 授权许可】

Unknown