Influence of Light Irradiation Rates and Irradiation Modes on Caffeine Production and Cell Growth in Suspension Culture of Coffea arabica Cells

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Tsutomu Furuya²  Minoru Seki¹  Hiroyuki Kurata¹  Shintaro Furusaki¹ 

[1] Department of Chemical Engineering, The University of Tokyo;School of Pharmaceutical Sciences, Kitasato University

关键词: Coffea arabica Cells;    Caffeine;    Irradiation Rates;    Irradiation Periods;    Light;    Two-Stage;    Intermittent Light Irradiation;    Chemical Actinometry;    Long-Term Cultivation;   

DOI  :  10.1252/jcej.24.783

来源: Maruzen Company Ltd

【 摘 要 】

References(14)Cited-By(4)The effect of light on caffeine production by Coffea arabica cells was investigated using chemical actinometry for the purpose of industrial application of plant cell cultures. Caffeine production by the Coffea arabica cells was greatly increased by light. About a six-day irradiation period was required to increase the caffeine production in a batch culture. Caffeine production significantly increased at a low light irradiation rate of 0.11 J/L/s. Meanwhile, cell growth was suppressed by increasing the irradiation rate. Maximum production was achieved at a moderate irradiation rate of 0.70 J/L/s in a 20-day batch culture. It was shown there was a light irradiation rate that optimized production. Since long-term continuous light greatly suppressed cell growth, the culture was divided into two stages: the production period in the light and the growth period in the dark. The high production and good growth were maintained for 65 days.

【 授权许可】

Unknown