Separation of Proteins by Continuous Isoelectric Focusing

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Shuang Ning Zheng¹  Toshikuni Yonemoto¹  Teiriki Tadaki¹ 

[1] Department of Biochemistry and Engineering, Tohoku University

关键词: Biochemical Engineering;    Isoelectric Focusing;    Separation;    Protein;    Albumin;    Hemoglobin;   

DOI  :  10.1252/jcej.24.471

来源: Maruzen Company Ltd

【 摘 要 】

References(10)Cited-By(1)An experimental investigation of continuous electrophoretic separation of bovine serum albumin from bovine hemoglobin was carried out. The separation behavior was analyzed with the model equations presented in our previous paper. We examined the effect of operating parameters such as applied voltage, residence time and the buffer added to feed solution including carrier ampholyte, on the basis of experimental results and theoretical predications, and showed that 1) an optimum applied voltage exists for the electrophoretic separation process of proteins, and 2) the buffer concentration greatly influences the pH distribution and the separation behavior of proteins.

【 授权许可】

Unknown