Growth Characteristics of Plant Hairy Roots and Their Cultures in Bioreactors

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Osamu Kondo¹  Chiaki Matsui²  Akihiko Yoyama¹  Masahito Taya¹  Takeshi Kobayashi¹ 

[1]Department of Chemical Engineering, Nagoya University

[2]Department of Agronomy, Nagoya University

关键词: Biochemical Engineering;    Plant Hairy Root;    Agrobacterium rhizogenes;    Immobilized Plant Cell;    Polyurethane Foam;    Conductivity Measurement;   

DOI  :  10.1252/jcej.22.84

来源: Maruzen Company Ltd

【 摘 要 】

References(26)Cited-By(44)Plant hairy roots from horseradish, carrots, etc. transformed by the soil bacterium Agrobacterium rhizogenes showed active growth in the phytohormone-free Murashige–Skoog medium with sucrose as a carbon source. Reticulate polyurethane foam was an appropriate support for the immobilization of hairy root cells. The hairy roots of 11 kg-dry cells·m–3 (110 kg-fresh cells·m–3) were obtained in a 31-d culture in an air-lift column bioreactor with immobilized horseradish cells. A linear relationship was observed between the dry cell mass grown and medium conductivity decrease for hairy root cultures. Thus, it was possible to monitor cell mass concentration during the hairy root culture by on-line measurement of conductivity in the bioreactor system.

【 授权许可】

Unknown