Plant Regeneration and Thiophene Production in Hairy Root Cultures of Rudbeckia hirta L. Used as an Antagonistic Plant to Nematodes

Japanese Journal of Crop Science

Masahiro MII¹  Hiroyuki DAIMON¹ 

关键词: Agrobacterium rhizogenes;    アグロバクテリウム リゾゲネス;    Antagonistic plant;    線虫;    Hairy root;    対抗植物;    Nematode;    チオフェン;    Rudbeckia hirta;    毛状根;    Thiophene;    ルドベキア;   

DOI  :  10.1626/jcs.64.650

学科分类：农业科学（综合）

来源: Nihon Sakumotsu Gakkai / Crop Science Society of Japan

【 摘 要 】

In Rudbeckia hirta L., an antagonistic plant to nematodes, hairy roots were induced by infection with a wild strain (A-5) of Agrobacterium rhizogenes. Hairy roots cultured in half-strength MS agar medium without phytohormones showed vigorous growth and extensive lateral branching. Mikimopine (opine) was detected in the extract of hairy root by paper electrophoresis. Adventitious shoots were induced on the surface of hairy roots after 30 to 50 days of transfer to half-strength MS agar medium supplemented with BAP at 0.5-10.0 mg/l. The highest frequency of shoot formation was obtained at 0.5 or 1.0 mg/l BAP in combination with 0.1mg/l NAA. Plants regenerated from hairy roots showed morphological alterations such as wrinkled leaves, small size of flowers and abundant lateral branching of roots. A nematocidal compound, α-terthienyl, was detected in the extract from lateral roots of the regenerant.

【 授权许可】

Unknown