Effect of Tocotrienol Rich Fraction (TRF) on synaptogenic molecules in aging Caenorhabdis elegans

International Journal of Biomedical and Advance Research

Goon Jo Aan¹  Norwahidah Abdul Karim¹  Ambhighavathe Siva Sarmugam¹ 

关键词: tocotrienol;    synapse;    aging;    C.elegans;   

DOI  :  10.7439/ijbar.v6i1.1583

学科分类：药学、药理学、毒理学（综合）

来源: Scholar Science Journals

【 摘 要 】

Objective:This study was performed to determine the effect of tocotrienol rich fraction (TRF) on the expression of synaptogenic genes and proteins in agingC.elegans . Method:Age synchronized DP132 strain worms were divided into two groups; control and treatment where worms were grown in nematode growth medium enriched with 50 ?g/ml TRF. The RNA and proteins expressions of syd-2, sad-1 and aex-3 were determined at day 2, 8 and 13 by q-RTPCR and western blot respectively. Results:The RNA and proteins expressions of syd-2, sad-1 and aex-3 decreased significantly with age inC.elegans . Interestingly, TRF treatment successfully increased the expression of these molecules from day 2 to day 8 which is known to be the reproductive phase ofC.elegansbut showed limited effect on the expression of these proteins at day 13. Conclusion:TRF enhances the expression of molecules involved in synaptic function of youngC.elegans .

【 授权许可】

CC BY