Cytokines profile and HLA typing in tolerant and non-tolerant patients to aspirin with nasossinusal polyposis

Revista Brasileira de Otorrinolaringologia

Nascimento, Evaldo¹  Crosara, Paulo F. T. B.¹  Universidade Federal de Minas Gerais¹  Becker, Celso G.¹  Gonçalves, Denise Utsch¹  Becker, Helena M. G.¹  Guimarães, Roberto E. S.¹ 

关键词: nasal polyps;    aspirin;    cytokines;    HLA antigen.;   

DOI  :  10.1590/S0034-72992003000300001

学科分类：医学（综合）

来源: Associacao Brasileira de Otorrinolaringologia e Cirurgia Cervicofacial

【 摘 要 】

The eosinophilic infiltration in the nasosinusal polyp associated with intolerance to aspirin is predominant feature. Several mediators play a role in the migration of the eosinophils to the tissues. The IA may be due to overexpression of leukotrienes in genetically susceptible subjects. AIM: The purpose of this study was to evaluate the cytokine pattern and HLA-A, B and DR typing in subjects with PNS tolerant and intolerants to aspirin. STUDY DESIGN: A transverse cohort study. MATERIAL AND METHOD: was conducted on 45 patients: 15 patients suffering from eosinophilic PNS and aspirin tolerance (group TA); 15 from eosinophilic PNS associated with aspirin intolerance, the latter manifested by bronchospasm (group IA), and 15 without PNS who had nasal septum deviation (control group). Cytokine pattern (IL-2; IL-4; IL-5; IL-6; IL-8; IL-10; IFN-g and TNF-a) was evaluated in samples from the nasal polyp or midlle turbinate mucosa (control group) of the patients using reverse transcription-polymerase chain reaction (RT-PCR). HLA-A, B and DR typing was performed using the serum microcytotoxicity test or by DNA amplification using polymerase chain reaction (PCR). RESULTS: mRNA expression for interleukines 4, 5, 6, 8, 10, IFN-g and TNF-a was similar in the three groups. mRNA expression for IL-2 was associated with IA. Patients with antigens A11, B49, DR15 and DR13 had a higher likelihood of developing PNS not-related to intolerance to Aspirin, whereas patients with DR17 had a higher likelihood of developing PNS associated with intolerance to Aspirin (Aspirin Triad). CONCLUSION: PNS associated with intolerance to Aspirin (Aspirin Triad) shows a significant association with HLA- DR17 and IL-2, suggesting a TH1-lymphocyte-activation pattern.

【 授权许可】

Unknown