Th17 cytokines expression and its correlation with periodontopathogens and periodontal inflammed surface area (PISA) in patients with chronic periodontitis

Revista clínica de periodoncia, implantología y rehabilitación oral

Sanz, A¹  Quintero, A¹  Universidad de los Andes, Chile¹  Herane, A¹  Hernández, M¹  Chaparro, A¹  Carrión, F¹  Gaedechens, D¹  Inostroza, C¹ 

关键词: Periodontitis;    PISA;    Th17;    periodontopathogens.;   

DOI  :  10.4067/S0719-01072013000300001

来源: Sociedad de Periodoncia de Chile

【 摘 要 】

Objective: Periodontitis is an infectious and inflammatory disease that involves a host immune response and is characterized by alveolar bone destruction. Aim: Analyze the expression of Th17 cytokines and their correlation with periodontopathogens and periodontal inflamed area in patients with chronic periodontitis. Method: A case control study was performed. At the time of delivery, 23 cases of patients with periodontal diagnosis were enrolled in the study and 10 controls with gingivitis. The diagnosis involved a complete periodontal examination with periodontal Florida probe. Also we used the PISA (Periodontal Inflamed Surface Area) index to classify the groups. Plasma and GCF samples were collected and studied for protein expression by ELISA assays for IL17A, Il6, IL23 and IL10. Plaque was analyzed by PCR for the determination of the presence of: P. gingivalis, T. denticola, T. forsythensis, F. nucleatum, P. intermedia and A. actinomycetemcomitans. Data was analyzed using descriptive statistics and the association between variables was estimated through logistic regression models. Results: There is a trend of increased GCF levels of IL17A, IL6, and IL23 with no significance. However there was an association between gingivitis and IL10 plasma and GCF levels (p=0.012). In relation to the periodontal wound size, a correlation was observed between the levels of IL6 and IL10 in GCF. Analysis of periodontal pathogens, showed an association between the presence of: P. gingivalis, T. denticola, T. forsythensis and plasma levels of IL-6 (p=0.017, 0.033 and 0.024, respectively).

【 授权许可】

Unknown