Quantification of caries-associated bacteria from saliva of adults and older adults

Revista clínica de periodoncia, implantología y rehabilitación oral

Muñoz-Sandoval, C¹  Universidad de Talca, Talca, Chile¹  Farfán-Cerda, P¹  Bravo González, E¹  Giacaman, RA¹ 

关键词: S. mutans;    S. sanguinis;    Lactobacillus spp.;    older adults;    caries;    oral biofilm.;   

DOI  :  10.4067/S0719-01072013000200004

来源: Sociedad de Periodoncia de Chile

【 摘 要 】

Introduction: Streptococcus mutans (S. mutans) and Lactobacillus spp. have been traditionally associated with caries, regardless of the subject’s age. Other oral biofilm species have been linked as caries protective, including Streptococcus sanguinis (S. sanguinis), but mainly in children. Scarce evidence exists on the levels of these bacteria in older adults. Aim: To determine whether there are differences in the microbial counts of three caries-associated bacterial species in adults and older adults. Methodology: A convenience sample of sixty three patients, aged 18 to 79 years, participated in the study. Stimulated saliva samples were obtained and in MSB, MM10 and Rogosa agar plates for the culture of S. mutans, S. sanguinis and Lactobacillus spp., respectively. Bacterial counts were obtained by microscopic observation (10x) of the colonies and expressed in CFU/mL. Results: Bacterial counts of S. mutans and Lactobacillus spp. did not reveal age-related differences (p>0.05). Adults showed higher S. sanguinis counts than older adults with 3.7 x 105 ± 3.8 x 105 CFU/mL and 5.9 x 104 ± 9.4 x 104 CFU/mL, respectively (p<0.05). Conclusions: Age does not seem to affect the levels of bacterial species traditionally associated with caries. The results suggest that age may be related to colonization patterns of S. sanguinis in the oral biofilm.

【 授权许可】

Unknown