Presence of markers of activation pathways of macrophages in chronic periodontitis

Revista clínica de periodoncia, implantología y rehabilitación oral

Puente, J¹  Universidad Andrés Bello, Viña del Mar, Chile¹  Dutzan, N¹  Gamonal, J¹  Navarrete, M¹  García-Sesnich, J¹  Universidad de Chile, Chile¹  Henríquez, L¹ 

关键词: Factor XIII-A;    macrophages;    periodontitis.;   

DOI  :  10.4067/S0719-01072012000300006

来源: Sociedad de Periodoncia de Chile

【 摘 要 】

Periodontitis is a chronic infectious disease caused by a bacterial species complex, which affects mainly the insertion tissues of the teeth. The immune-inflammatory response produced is characterized by an inflammatory infiltrate in which macrophages represent between 5 to 30%. It is known and has been widely discussed that macrophages are activated in two ways: Classical and Alterna, characterized by the presence of indirect markers: IFN-γ and IL-6 for the classical pathway and IL-4 for the alternative pathway. Recently the subunit A of the clotting factor XIII (FXIII-A) has been described as a good marker of the alternative pathway. The objective of this study is to determine the presence of IFN-γ, IL-6, IL-4 and FXIII-A as markers of the macrophage activation pathways in patients with chronic periodontitis. To this end, we performed immunohistochemistry and Western blot for the four markers with CD68  macrophage marker, in 18 healthy periodontal tissue biopsies and 18 with chronic periodontitis. We detected the presence of IFN-γ, IL-6, IL-4 and FXIII-A with CD68 +, in all samples of healthy patients and periodontitis. The results suggest that when present, IFN-γ, IL-6, IL-4 and FXIII-A, activate macrophages through both routes, which would produce a proinflammatory response (Th1) as antiinflammatory (Th2). Further studies are necessary to determine whether there is a preferential pathway activation.

【 授权许可】

Unknown