A STRATEGY FOR THE RAPID IDENTIFICATION OF FUNGAL METABOLITES AND THE DISCOVERY OF THE ANTIVIRAL ACTIVITY OF PYRENOCINE A AND HARZIANOPYRIDONE

Química Nova

Kohn, Luciana K.¹  Bandeira, Karin F.¹  Kossuga, Miriam H.¹  Universidade Estadual Paulista Júlio de Mesquita Filho, Rio Claro, Brazil¹  Rodrigues, Fabiana T.¹  Romminger, Stelamar¹  Universidade de São Francisco, Campinas, Brazil¹  Ferreira, Everton L. F.¹  University of Michigan, Ann Arbor, USA¹  Berlinck, Roberto G. S.¹  Sette, Lara D.¹  Passos, Messias S.¹  Santos, Mario F. C.¹  Universidade Estadual de Campinas, Campinas, Brazil¹  Arns, Clarice W.¹  Nicacio, Karen J.¹  Universidade de São Paulo, São Carlos, Brazil¹  Morais-Urano, Raquel P.¹  Ióca, Laura P.¹ 

关键词:  fungal metabolites;    rapid identification;    dereplication;    antiviral;   

DOI  :  10.5935/0100-4042.20160092

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The isolation and identification of bioactive metabolites from complex extracts obtained from microbial growth media is a time consuming, costly, and labor-intensive task. A strategy to rapidly identify secondary metabolites isolated from extracts obtained from the culture media of marine-derived and endophytic fungal strains is described. Identification was achieved by HPLC-UV-MS and 1H NMR analyses in combination with data obtained from the Dictionary of Natural Products. Among the compounds identified, (-)-naphthoquinoneimine, citreorosein, emodin, pyrenocine A and harzianopyridone displayed moderate to potent antiviral activity. (-)-Naphthoquinoneimine was isolated as the enantiomer of its previously reported dextrorotatory congener, while 6,7-dihydroxy-2,2-dimethyl-4-chromanone is herein reported for the first time as a natural product.

【 授权许可】

Unknown