Química Nova | |
A FACTORIAL DESIGN APPLIED TO THE STUDY OF CHROMIUM TOXICITY ON THE GLUTATHIONE LEVELS OF Brachiaria brizantha AND Brachiaria ruziziensis SEEDLINGS | |
Marques, Rafael1  Universidade Federal de Viçosa, Viçosa, BR1  Oliveira, Marcone A. L. de1  Passos, Leônidas P.1  Embrapa Pecuária Sul, Bagé, BR1  Universidade Federal de Juiz de Fora, Juiz de Fora, BR1  Reis, Cassia R. G. dos1  Embrapa Gado de Leite, Juiz de Fora, BR1  Köpp, Maurício M.1  | |
关键词: Â Brachiaria ruziziensis; Brachiaria brizantha; glutathione; chromium-toxicity; oxidative stress; | |
DOI : 10.5935/0100-4042.20150089 | |
学科分类:化学(综合) | |
来源: Sociedade Brasileira de Quimica | |
【 摘 要 】
Chromium toxicity affects redox reactions within plant cells, generating detrimental reactive oxygen species. Glutathione is an antioxidant peptide and also a substrate for the production of phytochelatins, which are chelating peptides reported to mitigate Cr3+ toxicity in plants. In this study, Brachiaria brizantha (B. brizantha) and Brachiaria ruziziensis (B. ruziziensis) seedlings were evaluated for physiological responses and glutathione production following the addition of zero or 5 mg L-1 Cr3+ to the nutrient solution. Glutathione levels were determined by colorimetric analysis at 412 nm using 5,5'-dithio-bis(2-nitrobenzoic acid) as a chromophore reagent and recovery with glutathione reductase (with evaluations at days 10 and 20 of continuous growth). The assessments were carried out in a completely randomized design with 2 authentic replications, and arranged in a 23 factorial. Cr3+ caused an average increase of 0.76 mg g-1 in the initial glutathione content. However, by day 20 there was an average reduction of 3.63 mg g-1. Chromium-affected physiological detrimental responses, albeit detected in both species, were less-pronounced in B. ruziziensis, along with a much higher level of glutathione. This study indicates that B. ruziziensis has a greater tolerance for chromium toxicity than B. brizantha, and that glutathione is likely to be involved in the mitigation of chromium stress in B. ruziziensis.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912050596580ZK.pdf | 652KB | download |