Evaluation of antiradical assays used in determining the antioxidant capacity of pure compounds and plant extracts

Química Nova

Universidade Presbiteriana Mackenzie, São Paulo, Brasil¹  Romoff, Paulete¹  Sobral, Edmar Silva¹  Souza, Glalci Alves de¹  Universidade de São Paulo, São Paulo, Brasil¹  Baader, Wilhelm Josef¹  Fávero, Oriana Aparecida¹  Eckert, Camila Rodrigues¹  Silva, Thuany Alves¹  Oliveira, Sandro de¹  Ferreira, Marcelo José Pena¹ 

关键词: luminol chemiluminescence;    antioxidant assays;    Trolox®;    percentage.;   

DOI  :  10.5935/0100-4042.20140076

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The efficiency of the chemiluminescence luminol method and colorimetric DPPH and ABTS methods in evaluating the antiradical capacity of pure compounds and plant extracts with antioxidant potential is compared. In case of pure compounds, the values of parameter 'n' (number of radicals quenched per molecule of antiradical) for ascorbic acid, p-hydroquinone, catechol, quercetin, and rutin are similar when measured by colorimetric assays; however, considerably lower values of n are obtained with the luminol assay. The antiradical activity of extracts from male and female individuals of Baccharis burchelli and Baccharis crispa were determined by the luminol assay and expressed using the new Trolox® percentage (%Trolox®) parameter.

【 授权许可】

Unknown