Evaluation of antiradical assays used in determining the antioxidant capacity of pure compounds and plant extracts

Quimica nova

Romoff, Paulete¹  Sobral, Edmar Silva¹  Fávero, Oriana Aparecida¹  Ferreira, Marcelo José Pena¹  Souza, Glalci Alves de²  Baader, Wilhelm Josef²  Eckert, Camila Rodrigues²  Silva, Thuany Alves²  Oliveira, Sandro de² 

[1] Universidade Presbiteriana Mackenzie, São Paulo, Brasil;Universidade de São Paulo, São Paulo, Brasil

关键词: luminol chemiluminescence;    antioxidant assays;    Trolox®;    percentage.;   

DOI  :  10.5935/0100-4042.20140076

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The efficiency of the chemiluminescence luminol method and colorimetric DPPH and ABTS methods in evaluating the antiradical capacity of pure compounds and plant extracts with antioxidant potential is compared. In case of pure compounds, the values of parameter 'n' (number of radicals quenched per molecule of antiradical) for ascorbic acid, p-hydroquinone, catechol, quercetin, and rutin are similar when measured by colorimetric assays; however, considerably lower values of n are obtained with the luminol assay. The antiradical activity of extracts from male and female individuals of Baccharis burchelli and Baccharis crispa were determined by the luminol assay and expressed using the new Trolox® percentage (%Trolox®) parameter.

【 授权许可】

CC BY