Validation of an UV spectrophotometric method for quantification of famotidine in capsules

Química Nova

Azevedo, Roberta de Cássia Pimentel¹  Bonfilio, Rudy¹  Araújo, Magali Benjamim de¹  Souza, Juliana Maria Oliveira¹  Universidade Federal de Alfenas, Alfenas, Brasil¹  Favoretto, Lívia Botacini¹ 

关键词: famotidine;    validation;    UV spectrophotometry.;   

DOI  :  10.1590/S0100-40422010000700028

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

This work describes the validation of a method for quantification of famotidine in capsules by UV spectrophotometry using as solvents buffer solution at pH 2.5 and buffer solution at pH 4.5. The results show that the method is practical, selective, accurate, precise and linear from 10.0 to 25.0 µg/mL of famotidine in both solvents. However, a variance analysis showed a lower mean percentage of famotidine when buffer at pH 2.5 is used. This effect was attributed to the instability of famotidine in acidic media. Therefore, the use of pH 4.5 buffer was considered suitable for analysis of famotidine in capsules.

【 授权许可】

Unknown