Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification

Química Nova

Farina, Marcelo¹  Garcia, Solange Cristina¹  Grotto, Denise¹  Maria, Lucas Santa¹  Pomblum, Valdeci Juarez¹  Universidade Federal de Santa Catarina, Florianópolis, Brasil¹  Rocha, João Batista T.¹  Schmitt, Gabriela¹  Universidade Federal de Santa Maria, Santa Maria, Brasil¹  Valentini, Juliana¹  Paniz, Clóvis¹ 

关键词: oxidative stress;    MDA;    methodologies.;   

DOI  :  10.1590/S0100-40422009000100032

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Free radicals induce lipid peroxidation, playing an important role in pathological processes. The injury mediated by free radicals can be measured by conjugated dienes, malondialdehyde, 4-hydroxynonenal, and others. However, malondialdehyde has been pointed out as the main product to evaluate lipid peroxidation. Most assays determine malondialdehyde by its reaction with thiobarbituric acid, which can be measured by indirect (spectrometry) and direct methodologies (chromatography). Though there is some controversy among the methodologies, the selective HPLC-based assays provide a more reliable lipid peroxidation measure. This review describes significant aspects about MDA determination, its importance in pathologies and biological samples treatment.

【 授权许可】

Unknown