Performance characteristics of bioassay UV-spectrophotometry and high perfomance liquid chromatographic determination of gatifloxacin in tablets

Química Nova

Lopes, Cristiani Capistrano Gonçalves de Oliveira¹  Salgado, Hérida Regina Nunes¹  Universidade Estadual Paulista, Araraquara, Brasil¹ 

关键词: fluoroquinolone;    gatifloxacin;    pharmaceutical analysis.;   

DOI  :  10.1590/S0100-40422008000700039

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

The microbiological bioassay, UV-spectrophotometry and HPLC methods for assaying gatifloxacin in tablets were compared. Validation parameters such as linearity, precision, accuracy, limit of detection and limit of quantitation were determined. Beer's law was obeyed in the ranges 4.0-14.0 μg/mL for HPLC and UV-spectrophotometric method, and 4.0-16.0 μg/mL for bioassay. All methods were reliable within acceptable limits for antibiotic pharmaceutical preparations being accurate, precise and reproducible. The bioassay and HPLC are more specific than UV-spectrophotometric analysis. The application of each method as a routine analysis should be investigated considering cost, simplicity, equipment, solvents, speed, and application to large or small workloads.

【 授权许可】

Unknown