Quantification of harman alkaloids in sour passion fruit pulp and seeds by a novel dual SBSE-LC/Flu (stir bar sorptive extraction-liquid chromatography with fluorescence detector) method

Journal of the Brazilian Chemical Society

Rodrigues, Thyago R.¹  Universidade de São Paulo, São Carlos, Brazil¹  Centro Universitário Central Paulista, São Carlos, Brazil¹  Pereira, Cíntia A. M.¹  Yariwake, Janete H.¹ 

关键词: dual SBSE-LC/Flu;    fractional factorial design;    harmane alkaloids;    sour passion fruit;   

DOI  :  10.5935/0103-5053.20140130

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

A method for the quantification of the alkaloids harmane and harmine in sour passion fruit (Passiflora edulis f. flavicarpa O. Degener, Passifloraceae) pulp and seeds by stir-bar sorptive extraction and high performance liquid chromatography with fluorescence detection (dual SBSE-LC/Flu) is described. The SBSE parameters were optimized using a fractional factorial design, and the dual SBSE-LC/Flu method was validated following the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) guidelines. The high sensitivity and minimal sample handling of the dual SBSE-LC/Flu method make it attractive for application in phytochemical analysis or in the food industry.

【 授权许可】

Unknown