Study of fatty acids profile in biological sample by capillary zone electrophoresis associate to chemometric approach

Journal of the Brazilian Chemical Society

Aarestrup, Beatriz J. V.¹  Oliveira, Marcone A. L.¹  Aragão, Danielle M. O.¹  Federal University of Santa Catarina,, Florianópolis, Brazil¹  Azevedo, Mônia S.¹  Barra, Patrícia M. C.¹  Federal University of Santa Catarina, Florianópolis, Brazil¹  Federal University of Juiz de Fora, Juiz de Fora, Brazil¹  Micke, Gustavo A.¹  Sabarense, Céphora M.¹  Oliveira, Patrícia L.¹  Costa, Ana Carolina O.¹ 

关键词: fatty acids;    liver rat;    capillary zone electrophoresis;    gas chromatography;    chemometric;   

DOI  :  10.5935/0103-5053.20140015

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

The determination of fatty acids (FA) in liver from three different groups of Wistar rats (six rats by group) submitted to diet (AIN-93G) by capillary zone electrophoresis (CZE) was proposed. Each group received the same diet. However, the soybean oil used to prepare the feed, whose amount was 7% w/w, had different origins. The first group was fed with feed containing soybean oil fresh; the second one was fed with soybean used during 7 days in deep frying process; finally, the third was fed with soybean used during 15 days in deep frying process. After 45 days the rats were submitted to euthanasia and the FA amount in liver was successful monitored by CZE in a simple, fast and efficient way. The results obtained were compared to gas chromatography official method and no significant differences were observed within 95% confidence interval. The electropherograms to FA analysis were submitted to principal component analysis (PCA) being possible to discriminate the final from the control and intermediate groups. In addition to PCA results, the low density lipoprotein (LDL) was evaluated indicating that the total time exposure of the oil submitted to deep frying processes can be considered relevant to evaluating the oil quality.

【 授权许可】

Unknown