Simultaneous analysis of saturated and unsaturated fatty acids present in pequi fruits by capillary electrophoresis

Química Nova

Patrícia M. De Castro Barra²  Marcone A. L. De Oliveira²  Bárbara Nery-enes¹  Leandro De M. Cardoso¹  Cristiane Do C. Cesário¹  Ana V. B. Moreira²  Helena M. Pinheiro-sant'ana²  Maria Do C. G. Peluzio¹ 

[1] ,Universidade Federal de Juiz de Fora Departamento de Química Juiz de Fora MG ,Brasil

关键词: pequi fruit;    gas chromatography;    capillary zone electrophoresis;   

DOI  :  10.1590/S0100-40422013000900025

来源: SciELO

【 摘 要 】

In the current study, an alternative method has been proposed for simultaneous analysis of palmitic, stearic, oleic, linoleic, and linolenic acids by capillary zone electrophoresis (CZE) using indirect detection. The background electrolyte (BGE) used for the analysis of these fatty acids (FAs) consisted of 15.0 mmol L−1 NaH2PO4/Na2HPO4 at pH 6.86, 4.0 mmol L−1 SDBS, 8.3 mmol L−1 Brij 35, 45% v/v acetonitrile (can), and 2.1% n-octanol. The FAs quantification of FAs was performed using a response factor approach, which provided a high analytical throughput for the real sample. The CZE method, which was applied successfully for the analysis of pequi pulp, has advantages such as short analysis time, absence of lipid fraction extraction and derivatization steps, and no significant difference in the 95% confidence intervals for FA quantification results, compared to the gas chromatography official method (AOCS Ce 1h-05).

【 授权许可】

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