Fast HPLC analysis of omeprazole, 5-hydroxyomeprazole and omeprazole sulfone in liquid culture medium using a monolithic column for application in biotransformation studies with fungi

Journal of the Brazilian Chemical Society

Pupo, Mônica T.¹  Universidad de Cádiz, Puerto Real, Spain¹  Sánchez, Antonio José M.¹  Durán-Patrón, Rosa¹  Universidade de São Paulo, Ribeirão Preto, Brazil¹  Collado, Isidro G.¹  Borges, Keyller B.¹  Bonato, Pierina S.¹ 

关键词: fast liquid chromatography;    monolithic column;    omeprazole;    5-hydroxyomeprazole;    omeprazole sulphone;    biotransformation;   

DOI  :  10.1590/S0103-50532011000600020

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

A fast liquid chromatography method was developed and validated for the simultaneous determination of omeprazole (OMZ), 5-hydroxyomeprazole (5-HOMZ) and omeprazole sulphone (OMZ SUL) in liquid culture medium for application in biotransformation studies employing phytopathogenic and endophytic fungi. The separation was achieved using a monolithic Chromolith Fast gradient RP 18 endcapped column, using a mobile phase consisting of 0.15% (v/v) trifluoroacetid acid (TFA) in water (solvent A) and 0.15% (v/v) TFA in acetonitrile (solvent B), under linear gradient of 5 to 90% of B in 1 min, flow rate of 1.0 mL min-1, temperature at 30 ÂºC and detection at 220 nm. Sample preparation was performed by liquid-liquid extraction, with recoveries in the range of 62.3 to 76.6% for all analytes. The method was linear in the range of 0.2 to 10.0 µg mL-1 (r ≥ 0.995). The values for intra- and inter-day precision (% coefficient of variation) and accuracy (% relative error) were < 15% for all analytes. The validated method was used to evaluate OMZ biotransformation to their mammalian metabolites by selected fungi. In general, the phytopathogenic fungi studied were more efficient to biotransform OMZ. The sulfonation reaction was more prevalent for all studied fungi.

【 授权许可】

Unknown