Determination of picloram in waters by sequential injection chromatography with UV detection

Journal of the Brazilian Chemical Society

Universidade do Estado da Bahia, Salvador, Brazil¹  Santos, Luciana B. O. dos¹  Infante, Carlos M. C.¹  Masini, Jorge C.¹  Universidade de São Paulo, São Paulo, Brazil¹ 

关键词: sequential injection chromatography;    monolithic column;    picloram;    waters;   

DOI  :  10.1590/S0103-50532010000800022

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

This paper describes a sequential injection chromatography procedure for determination of picloram in waters exploring the low backpressure of a 2.5 cm long monolithic C18 column. Separation of the analyte from the matrix was achieved in less than 60 s using a mobile phase composed by 20:80 (v v-1) acetonitrile:5.0 mmol L-1 H3PO4 and flow rate of 30 μL s-1. Detection was made at 223 nm with a 40 mm optical path length cell. The limits of detection and quantification were 33 and 137 μg L-1, respectively. The proposed method is sensitive enough to monitor the maximum concentration level for picloram in drinking water (500 μg L-1). The sampling frequency is 60 analyses per hour, consuming only 300 μL of acetonitrile per analysis. The proposed methodology was applied to spiked river water samples and no statistically significant differences were observed in comparison to a conventional HPLC-UV method.

【 授权许可】

Unknown