Synthesis and antimicrobial evaluation of some new pyrazole, pyrazoline and chromeno[3,4-c]pyrazole derivatives

Journal of the Brazilian Chemical Society

Al-Aqsa University, Gaza, Palestine¹  Hassaneen, Hamdi M.¹  Miqdad, Omar A.¹  Abunada, Nada M.¹  Samaha, Ahmed S. M. Abu¹  Cairo University, Cairo, Egypt¹ 

关键词: nitrilimines;    pyrazole;    pyrazoline;    chromeno[3;    4-c]pyrazole;   

DOI  :  10.1590/S0103-50532009000500024

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

Pyrazole and heterocyclic fused pyrazole derivatives represent an important class of heterocyclic compounds that have many applications. Some of these compounds are employed as anti-inflammatory compounds,1-3 as blood platelet aggregation inhibitors,1 as adenosine antagonists,4,5 and as controlling herbicides.6 They also show antimicrobial and antiparasitic activities.7,8 Also, pyrazoline derivatives have been found to possess antifungal,9 antidepressant10-13 anticonvulsant,12,13 antiinflammatory,14 antibacterial15 and anti-tumor16 activities. Chromenopyrazoles exhibit high activity against gram positive and gram negative bacteria.17 Moreover, many selectively fluoro-substituted organic compounds show a peculiar pharmacological and agrochemical properties.18-23 Therefore, as a connection of our interest in the chemistry of the preparation of heterocyclic compounds from hydrazonoyl halides24-29 and the above-mentioned findings, the present work is aimed at the preparation of new pyrazole, pyrazoline and their chromene fused derivatives incorporating fluorine and chlorine substituents into these derivatives hoping that it would potentiate their expected biological activities.  Experimental Hydrazonoyl bromides 130 and 231 were prepared by known methods. Melting points were measured on electrothermal melting point apparatus and are uncorrected. Elemental analyses were carried out at the Microanalytical Center of Cairo University, Giza, Egypt. Infrared spectra were recorded in potassium bromide pellets on a Pye Unicam SP 3-300 and Shimadzu FT-IR 8101 PC infrared spectrophotometer. NMR spectra were recorded at 200 (1H) and 50 (13C) MHz in (DMSO-d6) on a GEMINI-200 spectrometer. Chemical shifts (d) are reported relative to TMS as the internal standard. Mass spectra were measured on a GCMS-QP 1000 EX spectrometer operating at an ionization potential of 70 eV.Synthesis of 3,4-diaryl-1-(4-nitrophenyl)-1H-pyrazole-5-carbonitriles 8, 9 Triethylamine (0.7 mL, 5 mmol) was added to a stirred solution of the appropriate hydrazonoyl bromides 1, 2 and the appropriate α-cyanocinnamonitrile derivatives 5a-f (5 mmol) in benzene (40 mL) at room temperature. The mixture was refluxed for 8 h as indicated by TLC. The precipitated triethylamine hydrobromide was removed by filtration and the filtrate was evaporated, and then triturated with methanol. The solid that formed was collected by filtration and crystallized from the suitable solvent to give 8, 9 respectively. 3-(4-Fluorophenyl)-1-(4-nitrophenyl)-4-phenyl-1H-pyrazole-5-carbonitrile 8a Obtained as pale yellow crystals; yield: 1.07 g (56%); mp214-6 ºC (from dioxane-ethanol); IR (KBr)vmax/cm-1: 3119.0, 3083.1 (CH-aromatic), 2235.5 (C≡N), 1659.2 (C=N), 1592.7 (C=C); 1H NMR (DMSO-d6) d7.04-8.54 (m, 13H, ArH's); 13C NMR (DMSO-d6) d161.28 (d, J 256.4 Hz, C-F), 146.38 , 146.14 (C=N, C-p-NO2), 143.10, 138.83, 135.12, 129.76 (d, J 8.3 Hz, C-m-F), 129.25, 127.97, 125.72, 126.52, 125.14, 124.86 (d, J 3.1 Hz, C-p-F), 124.71, 115.50 (d, J 22.1 Hz, C-o-F), 110.61 (C≡N); MS, m/z: 385 (M++1, 88.8), 384 (M+, 100.0), 338 (10.3), 337 (15.0), 196 (10.4), 75 (14.5), 63 (13.4). Anal. Calc. for C22H13FN4O2 (Mr = 384.36): C, 68.74; H, 3.40; N, 14.57%; Found: C, 68.62; H, 3.39; N, 14.34%.3-(2,4-Dichlorophenyl)-1-(4-nitrophenyl)-4-phenyl-1H-pyrazole-5-carbonitrile 9a Obtained as off white solid; yield: 1.13 g (52%); mp 160-162 ºC (from acetic acid); IR (KBr)vmax/cm-1: 3085.3 (CH-aromatic), 2228.1 (C≡N), 1652.5 (C=N), 1595.1 (C=C); 1H NMR (DMSO-d6) d 7.01-8.54 (m, 12H, ArH's); 13C NMR (DMSO-d6) d147.30, 146.38 (C=N, C-p-NO2), 145.14, 138.83, 135.12, 133.36, 132.45, 130.33, 130.08, 129.98, 129.45, 129.25, 127.97, 125.72, 126.52, 125.14, 124.71, 110.61 (C≡N); MS, m/z: 436 (M++2, 46.3), 434 (M+, 68.1), 401 (38.4), 399 (100.0), 355 (16.4), 353 (48.3), 319 (6.3), 317 (18.7), 246 (17.0), 216 (15.0), 190 (18.6), 127 (23.5), 75 (53.1), 50 (39.2). Anal. Calc. for C22H12Cl2N4O2 (Mr = 435.26): C, 60.70; H, 2.77; N, 12.87; Cl, 16.29%; Found: C, 60.58; H, 2.87; N, 12.69; Cl, 16.31%. 3-(4-Fluorophenyl)-4-(4-methylphenyl)-1-(4-nitrophenyl)-1H-pyrazole-5-carbonitrile 8b Obtained as off white solid; yield: 1.01 g (51%); mp 163-165ºC (from acetic acid); IR (KBr)vmax/cm-1: 3116.1, 3080.6 (CH-aromatic), 2924.1, 2854.2 (CH-aliphatic), 2233.1 (C≡N), 1650.6 (C=N), 1597.0 (C=C); 1H NMR (DMSO-d6) d 7.02-8.58 (m, 12H, ArH's), 2.25 (s, 3H, CH3); 13C NMR (DMSO-d6) δ 161.30 (d, J 256.4 Hz, C-F), 146.39, 146.10 (C=N, C-p-NO2), 143.13, 138.77, 135.09, 129.78 (d, J 8.3 Hz, C-m-F), 129.28, 128.48, 126.92, 126.51, 125.22, 124.88 (d, J 3.1 Hz, C-p-F), 122.85, 115.52 (d, J 22.1 Hz, C-o-F), 110.58 (C≡N), 20.39 (CH3); MS, m/z: 399 (M++1, 71.4), 398 (M+, 100.0), 383 (13.5), 351 (10.8), 76 (14.9), 75 (23.2). Anal. Calc. for C23H15FN4O2 (Mr = 398.38): C, 69.34; H, 3.79; N, 14.06%; Found: C, 69.14; H, 3.82; N, 13.96%.3-(2,4-Dichlorophenyl)-4-(4-methylphenyl)-1-(4-nitrophenyl)-1H-pyrazole-5-carbonitrile 9b Obtained as off white solid; yield: 1.16 g (52%); mp 195-197 ºC (from acetic acid); IR (KBr)vmax/cm-1: 3081.2 (CH-aromatic), 2920.3, 2854.8 (CH-aliphatic), 2226.9 (C≡N), 1653.1 (C=N), 1595.0 (C=C); 1H NMR (DMSO-d6) δ 7.02-8.54 (m, 11H, ArH's), 2.26 (s, 3H, CH3); 13C NMR (DMSO-d6) δ 147.32, 146.39 (C=N, C-p-NO2), 146.10, 138.77, 135.09, 133.40, 132.44, 130.30, 130.10, 129.96, 129.43, 129.28, 128.48, 126.92, 126.51, 125.22, 122.85 (15C, ArC's), 110.58 (C≡N), 20.36 (CH3); MS, m/z: 450 (M++2, 76.8), 448 (M+, 100.0), 413 (82.8), 378 (48.1), 367 (42.6), 257 (10.9), 230 (12.6), 165 (11.2), 140 (27.8), 103 (14.7), 90 (21.7), 75 (49.1). Anal. Calc. for C23H14Cl2N4O2 (Mr = 449.28): C, 61.48; H, 3.14; N, 12.47; Cl, 15.78%; Found: C, 61.42; H, 3.21; N, 12.39; Cl, 15.69%. 3-(4-Fluorophenyl)-4-(4-methoxyphenyl)-1-(4-nitrophenyl)-1H-pyrazole-5-carbonitrile 8c Obtained as off white solid; yield: 1.01 g (49%); mp 194-196ºC (from acetic acid); IR (KBr)vmax/cm-1: 3120.7, 3089.4 (CH-aromatic), 2936.5 (CH-aliphatic), 2231.1 (C≡N), 1651.8 (C=N), 1598.6 (C=C); 1H NMR (DMSO-d6) δ 7.03-8.58 (m, 12H, ArH's), 3.77 (s, 3H, OCH3); 13C NMR (DMSO-d6) δ 161.31 (d, J 256.4 Hz, C-F), 160.82 (C-OCH3), 146.39, 146.10 (C=N, C-p-NO2), 143.12, 135.11, 129.78 (d, J 8.3 Hz, C-m-F), 129.67, 129.27, 126.51, 125.22, 124.86 (d, J 3.1 Hz, C-p-F), 116.23, 115.52 (d, J 22.1 Hz, C-o-F), 114.54, 110.58 (C≡N), 55.18 (OCH3); MS, m/z: 414 (M+, 100.0), 399 (9.3), 75 (19.8), 74 (17.9). Anal. Calc. for C23H15FN4O3 (Mr = 414.38): C, 66.66; H, 3.64; N, 13.52%; Found: C, 66.43; H, 3.61; N, 13.49%. 3-(2,4-Dichlorophenyl)-4-(4-methoxyphenyl)-1-(4-nitrophenyl)-1H-pyrazole-5-carbonitrile 9c Obtained as off white solid; yield: 1.11 g (48%); mp 147-149 ºC (from acetic acid); IR (KBr)vmax/cm-1: 3082.6 (CH-aromatic), 2933.8 (CH-aliphatic), 2224.6 (C≡N), 1652.4 (C=N), 1596.6 (C=C); 1H NMR (DMSO) δ 7.01-8.55 (m, 11H, ArH's), 3.78 (s, 3H, OCH3); 13C NMR (DMSO-d6) δ 159.33 (C-OCH3), 148.02, 146.50 (C=N, C-p-NO2), 141.91, 134.69, 133.41, 133.21, 131.43, 129.00, 128.84, 128.43, 127.39, 124.94, 122.99, 119.88, 114.20, 112.15 (14C, ArC's), 110.78 (C≡N), 54.75 (OCH3); MS, m/z: 466 (M++2, 62.1), 464 (M+, 100.0), 394 (17.5), 76 (18.2), 75 (41.7), 63 (17.4), 62 (16.4), 50 (17.7). Anal. Calc. for C23H14Cl2N4O3 (Mr = 465.28): C, 59.37; H, 3.03; N, 12.04; Cl, 15.23%; Found: C, 59.15; H, 3.11; N, 12.14; Cl, 15.31%. 4-(4-Chlorophenyl)-3-(4-fluorophenyl)-1-(4-nitrophenyl)-1H-pyrazole-5-carbonitrile 8d Obtained as off white solid; yield: 1.06 g (51%); mp 160-2 ºC (from acetic acid); IR (KBr)vmax/cm-1: 3113.3, 3079.5 (CH-aromatic), 2231.6 (C≡N), 1656.3 (C=N), 1594.6 (C=C) ; 1H NMR (DMSO-d6) δ 7.03-8.54 (m, 12H, ArH's); 13C NMR (DMSO-d6) δ 161.33 (d, J 256.4 Hz, C-F), 146.42 , 146.16 (C=N, C-p-NO2), 143.18, 137.55, 135.11, 130.09, 129.76 (d, J 8.3 Hz, C-m-F), 128.88, 126.89, 126.50, 125.20, 124.88 (d, J 3.1 Hz, C-p-F), 121.05, 115.53 (d, J 22.1 Hz, C-o-F), 110.61 (C≡N); MS, m/z: 419 (M++1, 84.1), 418 (M+, 100.0), 371 (10.9), 338 (12.4), 230 (12.6), 95 (10.7), 75 (26.0), 63 (15.7), 50 (19.2). Anal. Calc. for C22H12ClFN4O2 (Mr = 418.80): C, 63.09; H, 2.88; N, 13.37; Cl, 8.46%; Found: C, 62.89; H, 2.94; N, 13.29; Cl, 8.52%.4-(4-Chlorophenyl)-3-(2,4-dichlorophenyl)-1-(4-nitrophenyl)-1H-pyrazole-5-carbonitrile 9d Obtained as white solid; yield: 1.10 g (47%); mp 168-170ºC (from acetic acid); IR (KBr)vmax/cm-1: 3087.7 (CH-aromatic), 2228.7 (C≡N), 1655.3 (C=N), 1596.6 (C=C); 1H NMR (DMSO-d6) δ 7.32-8.56 (m, 11H, ArH's); 13C NMR (DMSO-d6) δ 148.08, 146.42 (C=N, C-p-NO2), 146.16, 137.55, 135.11, 133.45, 132.67, 131.87, 130.62, 130.09, 129.87, 129.74, 128.88, 126.89, 126.50, 125.20, 121.05 (15C, ArC's), 110.61 (C≡N); MS, m/z: 470 (M++2, 100.0), 468 (M+, 92.1), 433 (56.1), 400 (36.8), 387 (41.9), 386 (21.3), 317 (28.1), 289 (19.4), 277 (45.5), 216 (28.9), 177 (22.1), 163 (20.9), 136 (23.3), 100 (26.9), 90 (22.5), 75 (89.7), 63 (83.0), 51 (41.5), 50 (96.8). Anal. Calc. for C22H11Cl3N4O2 (Mr = 469.70): C, 56.25; H, 2.36; N, 11.92; Cl, 22.64%; Found: C, 56.29; H, 2.35; N, 11.94; Cl, 22.66%. 3,4-Di-(4-fluorophenyl)-1-(4-nitrophenyl)-1H-pyrazole-5-carbonitrile 8e Obtained as yellow crystals; yield: 0.96 g (48%); mp 178-180 ºC (from acetic acid); IR (KBr)vmax/cm-1: 3114.6, 3079.9 (CH-aromatic), 2231.8 (C≡N), 1657.1 (C=N), 1595.1 (C=C); 1H NMR (DMSO-d6) δ 7.04-8.58 (m, 12H, ArH's); 13C NMR (DMSO-d6) δ 164.40 (d, J 255.8 Hz, C-F), 161.30 (d, J 256.4 Hz, C-F), 146.39 , 146.10 (C=N, C-p-NO2), 143.13, 135.09, 132.74 (d, J 8.2 Hz, C-m-F), 129.78 (d, J 8.3 Hz, C-m-F), 129.2, 126.51, 125.22, 124.88 (d, J 3.1 Hz, C-p-F), 123.72 (d, J 3.2 Hz, C-p-F), 116.89 (d, J 22.4 Hz, C-o-F), 115.52 (d, J 22.1 Hz, C-o-F), 110.58 (C≡N); MS, m/z: 403 (M++1, 98.3), 402 (M+, 100.0), 356 (10.0), 355 (12.6), 214 (11.9), 75 (19.6). Anal. Calc. for C22H12F2N4O2 (Mr = 402.35): C, 65.67; H, 3.00; N, 13.92%; Found: C, 65.46; H, 2.96; N, 13.87%. 3-(2,4-Dichlorophenyl)-4-(4-fluorophenyl)-1-(4-nitrophenyl)-1H-pyrazole-5-carbonitrile 9e Obtained as off white solid; yield: 1.04 g (46%); mp 170-172 ºC (from acetic acid); IR (KBr)vmax/cm-1: 3113.3, 3087.4 (CH-aromatic), 2228.1 (C≡N), 1655.9 (C=N), 1596.4 (C=C); 1H NMR (DMSO-d6) δ 7.31-8.59 (m, 11H, ArH's); 13C NMR (DMSO-d6) δ 164.40 (d, J 255.8 Hz, C-F), 148.47, 146.39 , 146.10 (C=N, C-p-NO2), 135.09, 133,65, 132.46, 132.74 (d, J 8.2 Hz, C-m-F), 130.45, 130.21, 129.88, 129.48, 129.2, 126.51, 125.22, 123.72 (d, J 3.2 Hz, C-p-F), 115.52 (d, J 22.1 Hz, C-o-F), 110.58 (C≡N); MS, m/z: 454 (M++2, 80.8), 452 (M+, 100.0), 417 (63.0), 371 (46.8), 335 (16.2), 264 (31.8), 208 (37.0), 145 (35.2), 107 (30.5), 75 (54.7), 63 (45.3), 50 (51.0). Anal. Calc. for C22H11Cl2FN4O2 (Mr = 453.25): C, 58.29; H, 2.44; N, 12.36; Cl, 15.64%; Found: C, 58.31; H, 2.46; N, 11.34; Cl, 15.66%. 3-(2,4-Dichlorophenyl)-1,4-di-(4-nitrophenyl)-1H-pyrazole-5-carbonitrile 9f Obtained as brown crystals; yield: 1.05 g (44%); mp 220-222 ºC (from acetic acid); IR (KBr)vmax/cm-1: 3086.9 (CH-aromatic), 2233.7 (C≡N), 1594.8 (C=C); 1H NMR (DMSO-d6) δ 7.58-8.56 (m, 11H, ArH's); 13C NMR (DMSO-d6) δ 148.22, 147.37, 146.46 (C=N, 2C-p-NO2), 145.24, 135.56, 134.02, 133.89, 131.15, 130.31, 130.16, 129.94, 129.38, 128.64, 128.34, 126.78, 125.42, 119.78 (14C, ArC's), 110.61 (C≡N); MS, m/z: 481 (M++2, 80.8), 479 (M+, 100.0), 398 (92.3), 351 (21.8), 317 (18.5), 215 (21.5), 76 (28.1), 75 (38.1), 63 (29.6), 50 (37.2). Anal. Calc. for C22H11Cl2N5O4 (Mr = 480.25): C, 55.01; H, 2.47; N, 14.64; Cl, 14.76%; Found: C, 55.00; H, 2.46; N, 14.65; Cl, 14.77%. Synthesis of 3,4-diaryl-1-(4-nitrophenyl)-1H-pyrazole-5-carboxamides 13 This reaction was carried out by the same method described for the preparation of the previous pyrazoles 8, 9 using α-cyanocinnamamide derivatives 12a, b in place of α-cyanocinnamonitrile derivatives 5a-f. The prepared compounds 13a, b together with their physical and spectral data are listed below. 3-(2,4-Dichlorophenyl)-1-(4-nitrophenyl)-4-phenyl-1H-pyrazole-5-carboxamide 13a Obtained as off white solid; yield: 1.04 g (46%); mp 233-235 ºC (from acetic acid); IR (KBr)vmax/cm-1: 3354.9, 3254.1 (NH2), 3083.4 (CH-aromatic), 1669.6 (C=O amide), 1627.2 (C=N), 1597.0 (C=C); 1H NMR (DMSO-d6) δ 7.18-8.46 (m, 14H, ArH's, NH2); 13C NMR (DMSO-d6) δ 167.78 (C=O amide), 149.38, 145.63 (C=N, C-p-NO2), 145.12, 133.38, 132.39, 130.03, 129.82, 129.77, 128.39, 127.78, 127.67, 127.02, 126.69, 125.09, 124.87, 124.38, 123.78 (15C, ArC's); MS, m/z: 454 (M++2, 65.1), 452 (M+, 95.4), 400 (77.8), 354 (51.6), 356 (25.8), 351 (33.7), 190 (26.6), 168 (19.8), 163 (21.2), 115 (26.2), 90 (22.0), 89 (100.0), 76 (36.6), 63 (33.9), 50 (34.5). Anal. Calc. for C22H14Cl2N4O3 (Mr = 453.27): C, 58.29; H, 3.11; N, 12.36; Cl, 15.64%; Found: C, 58.31; H, 3.12; N, 12.38; Cl, 15.66%. 3-(2,4-Dichlorophenyl)-1,4-di-(4-nitrophenyl)-1H-pyrazole-5-carboxamide 13b Obtained as pale yellow solid; yield: 1.12 g (45%); mp 182-184 ºC (from acetic acid); IR (KBr)vmax/cm-1: 3301.4, 3228.6 (NH2), 3117.5, 3092.1 (CH-aromatic), 1711.0 (C=O amide), 1591.0 (C=C); 1H NMR (DMSO-d6) δ 7.42-8.52 (m, 11H, ArH's), 6.25 (s, br., 2H, NH2); 13C NMR (DMSO-d6) δ 167.77 (C=O amide), 149.72, 145.61 (C=N, C-p-NO2), 145.40, 136.16, 133.38, 132.32, 129.82, 129.79, 128.39, 127.76, 127.53, 127.09, 126.31, 126.23, 124.89, 124.37, 121.78 (15C, ArC's), 21.38 (CH3); MS, m/z: 499 (M++2, 71.5), 497 (M+, 100.0), 462 (12.6), 418 (13.5), 416 (30.5), 362 (16.9), 360 (12.4), 190 (16.0), 88 (11.3), 76 (19.3), 63 (14.4), 50 (17.1). Anal. Calc. for C22H13Cl2N5O5 (Mr = 498.27): C, 53.02; H, 2.62; N, 14.05; Cl, 14.23%; Found: C, 53.00; H, 2.64; N, 14.07; Cl, 14.22%. Synthesis of ethyl 5-cyano-3,4-diaryl-1-(4-nitrophenyl)-2-pyrazoline-5-carboxylates 15, 16 This reaction was carried out by the same method described for the preparation of the previous pyrazoles 8, 9 using ethyl α-cyanocinnamate derivatives 14a-f in place of α-cyanocinnamonitrile derivatives 5a-f. Compounds 15, 16 with their physical and spectral data are listed below. Ethyl 5-cyano-3-(4-fluorophenyl)-1-(4-nitrophenyl)-4-phenyl-2-pyrazoline-5-carboxylate 15a Obtained as yellow crystals; yield: 1.19 g (52%); mp 214-215 ºC (from acetic acid); IR (KBr)vmax/cm-1: 3107.7, 3088.4 (CH-aromatic), 2984.3 (CH-aliphatic), 1762.6 (C=O ester), 1592.9 (C=C); 1H NMR (DMSO-d6) d 7.13-8.42 (m, 13H, ArH's), 6.20 (s, 1H pyrazoline), 4.08 (q, 2H, J 7.0 Hz, COOCH2CH3), 0.93 (t, 3H, J 7.0 Hz, COOCH2CH3); 13C NMR (DMSO-d6) δ 165.17 (d, J 256.1 Hz, C-F), 164.38 (C=O ester), 148.26, 146.25 (C=N, C-p-NO2), 143.43, 132.65, 130.40, 129.37, 129.20 (d, J 8.3 Hz, C-m-F), 127.25 (d, J 3.1 Hz, C-p-F), 127.04, 124.73, 120.97, 115.97 (d, J 22.2 Hz, C-o-F), 111.02 (C≡N), 70.92 (C-5 pyrazoline), 63.35 (C-4 pyrazoline), 61.31 (OCH2CH3), 12.91 (OCH2CH3); MS, m/z: 459 (M++1, 17.4), 458 (M+, 17.0), 386 (100.0), 385 (94.0), 340 (21.2), 339 (21.9), 90 (5.3), 76 (6.6), (21.9), 90 (5.3), 76 (6.6), 51 (4.5), 50 (4.6). Anal. Calc. for C25H19FN4O4 (Mr = 458.44): C, 65.49; H, 4.17; N, 12.22 ; Found: C, 65.56; H, 4.26; N, 12.45%. Ethyl 5-cyano-3-(2,4-dichlorophenyl)-1-(4-nitrophenyl)-4-phenyl-2-pyrazoline-5-carboxylate 16a Obtained as yellow crystals; yield: 1.29 g (51%); mp 203-204 ºC (from acetic acid); IR (KBr)vmax/cm-1: 3066.3 (CH-aromatic), 2995.8, 2926.0 (CH-aliphatic), 1742.7 (C=O ester), 1655.0 (C=N), 1592.6 (C=C); 1H NMR (DMSO-d6) δ 7.12-8.43 (m, 12H, ArH's), 6.21 (s, 1H pyrazoline), 4.17 (q, 2H, J 7.2 Hz, COOCH2CH3), 0.95 (t, 3H, J 7.2 Hz, COOCH2CH3); 13C NMR (DMSO-d6) d 163.39 (C=O ester), 148.58, 146.13 (C=N, C-p-NO2), 143.83, 134.05, 133.82, 133.26, 132.48, 130.37, 129.47, 129.17, 128.25, 127.08, 126.88, 124.96, 121.78 (13C, ArC's), 112.98 (C≡N), 70.71 (C-5 pyrazoline), 64.25 (C-4 pyrazoline), 61.24 (OCH2CH3), 12.84 (OCH2CH3); MS, m/z: 510 (M++2, 9.8), 508 (M+, 11.2), 437 (62.4), 435 (100.0), 391 (14.0), 389 (24.7), 219 (8.4), 218 (11.0), 190 (10.4), 90 (15.0), 89 (15.9), 77 (17.0), 76 (20.9), 75 (12.9), 63 (19.0), 51 (15.9), 50 (16.6). Anal. Calc. for C25H18Cl2N4O4 (Mr = 509.34): C, 58.95; H, 3.56; N, 10.99; Cl, 13.92%; Found: C, 58.83; H, 3.58; N, 11.02; Cl, 14.01%. Ethyl 5-cyano-3-(4-fluorophenyl)-4-(4-methylphenyl)-1-(4-nitrophenyl)-2-pyrazoline-5-carboxylate 15b Obtained as yellow crystals; yield: 1.27 g (54%); mp 187-190 ºC (from acetic acid); IR (KBr)vmax/cm-1: 3188.7 (CH-aromatic), 2983.3, 2961.1 (CH-aliphatic), 1747.1 (C=O ester), 1589.0 (C=C); 1H NMR (DMSO-d6) d 7.14-8.44 (m, 12H, ArH's), 6.21 (s, 1H pyrazoline), 4.07 (q, 2H, J 7.0 Hz, COOCH2CH3), 2.26 (s, 3H, CH3), 0.91 (t, 3H, J 7.0 Hz, COOCH2CH3); 13C NMR (DMSO-d6) d 165.17 (d, J 256.1 Hz, C-F), 164.38 (C=O ester), 148.26, 146.25 (C=N, C-p-NO2), 143.43, 137.86, 130.88, 129.20 (d, J 8.3 Hz, C-m-F), 128.16, 127.25 (d, J 3.1 Hz, C-p-F), 126.54, 124.73, 120.99, 115.97 (d, J 22.2 Hz, C-o-F), 111.02 (C≡N), 70.92 (C-5 pyrazoline), 63.35 (C-4 pyrazoline), 61.31 (OCH2CH3), 21.06 (CH3), 12.91 (OCH2CH3); MS, m/z: 473 (M++1, 11.7), 472 (M+, 13.8), 400 (89.4), 399 (100.0), 354 (17.6), 353 (20.8), 76 (12.3), 63 (10.7). Anal. Calc. for C26H21FN4O4 (Mr = 472.46): C, 66.09; H, 4.48; N, 11.85%; Found: C, 65.89; H, 4.39; N, 11.76%.Ethyl 5-cyano-3-(2,4-dichlorophenyl)-4-(4-methylphenyl)-1-(4-nitrophenyl)-2-pyrazoline-5-carboxylate 16b Obtained as yellow crystals; yield: 1.36 g (52%); mp 200-203 ºC (from acetic acid); IR (KBr)vmax/cm-1: 3063.7 (CH-aromatic), 2982.3 (CH-aliphatic), 1761.1 (C=O ester), 1651.8 (C=N); 1H NMR (DMSO-d6) δ 7.11-8.42 (m, 11H, ArH's), 6.21 (s, 1H, pyrazoline), 4.11-4.21 (q, 2H, J 7.2 Hz, COOCH2CH3), 2.27 (s, 3H, CH3), 0.98 (t, 3H, J 7.2 Hz, COOCH2CH3); 13C NMR (DMSO-d6) δ 163.37 (C=O ester), 148.61, 146.15 (C=N, C-p-NO2), 143.86, 135.66, 134.10, 133.28, 132.45, 130.89, 130.34, 129.48, 129.16, 127.03, 126.82, 124.96, 121.81 (13C, ArC's), 112.86 (C≡N), 70.82 (C-5 pyrazoline), 64.31 (C-4 pyrazoline), 61.27 (OCH2CH3), 21.34 (CH3), 12.84 (OCH2CH3); MS, m/z: 522 (M+, 14.1), 451 (81.4), 449 (100.0), 403 (18.0), 76 (18.0), 75 (15.9), 63 (13.9), 62 (13.3), 50 (12.2). Anal. Calc. for C26H20Cl2N4O4 (Mr = 523.36): C, 59.66; H, 3.85; N, 10.70; Cl, 13.54%; Found: C, 59.58; H, 3.82; N, 10.68; Cl, 13.49%. Ethyl 5-cyano-3-(4-fluorophenyl)-4-(4-methoxyphenyl)-1-(4-nitrophenyl)-2-pyrazoline-5-carboxylate 15c Obtained as yellow crystals; yield: 1.19 g (49%); mp 183-184 ºC (from acetic acid); IR (KBr)vmax/cm-1: 3076.8 (CH-aromatic), 2983.3 (CH-aliphatic), 1764.5 (C=O ester), 1597.7 (C=C); 1H NMR (DMSO-d6) δ 7.14-8.43 (m, 12H, ArH's), 6.20 (s, 1H pyrazoline), 4.08 (q, 2H, J 7.2 Hz, COOCH2CH3), 3.76 (s, 3H, OCH3), 0.90 (t, 3H, J 7.2 Hz, COOCH2CH3); 13C NMR (DMSO-d6) δ 165.17 (d, J 256.1 Hz, C-F), 164.38 (C=O ester), 158.10 (C-OCH3), 148.26, 146.25 (C=N, C-p-NO2), 143.43, 129.20 (d, J 8.3 Hz, C-m-F), 128.23, 127.25 (d, J 3.1 Hz, C-p-F), 126.54, 124.73, 123.17, 121.10, 115.97 (d, J 22.2 Hz, C-o-F), 111.02 (C≡N), 70.92 (C-5 pyrazoline), 63.35 (C-4 pyrazoline), 61.31 (OCH2CH3), 54.40 (OCH3), 12.91 (OCH2CH3); MS, m/z: 489 (M++1, 5.4), 488 (M+, 13.1), 416 (91.5), 415 (100.0), 370 (4.1), 369 (14.2), 77 (10.9), 75 (9.6). Anal. Calc. for C26H21FN4O5 (Mr = 488.46): C, 63.92; H, 4.33; N, 11.47%; Found: C, 63.79; H, 4.26; N, 11.41%. Ethyl 5-cyano-3-(2,4-dichlorophenyl)-4-(4-methoxyphenyl)-1-(4-nitrophenyl)-2-pyrazoline-5-carboxylate 16c Obtained as yellow crystals; yield: 1.26 g (47%); mp 182-184ºC (from acetic acid); IR (KBr)vmax/cm-1: 3071.8 (CH-aromatic), 2974.7 (CH-aliphatic), 1763.4 (C=O ester), 1651.1 (C=N), 1591.7 (C=C); 1H NMR (DMSO-d6) δ 7.13-8.44 (m, 11H, ArH's), 6.23 (s, 1H pyrazoline), 4.18 (q, 2H, J 7.2 Hz, COOCH2CH3), 3.77 (s, 3H, OCH3), 0.96 (t, 3H, J 7.2 Hz, COOCH2CH3); 13C NMR (DMSO-d6) δ 163.39 (C=O ester), 158.79 (C-OCH3), 148.58, 146.13 (C=N, C-p-NO2), 143.83, 134.05, 133.26, 132.48, 130.37, 129.47, 128.63, 126.88, 126.49, 124.96, 124.03, 121.78 (12C, ArC's), 112.98 (C≡N), 70.71 (C-5 pyrazoline), 64.25 (C-4 pyrazoline), 61.24 (OCH2CH3), 54.50 (OCH3), 12.84 (OCH2CH3); MS, m/z: 540 (M++2, 10.9), 538 (M+, 14.1), 467 (70.1), 465 (100.0), 421 (8.2), 419 (13.0), 77 (8.4), 76 (11.1), 63 (8.8), 50 (7.4). Anal. Calc. for C26H20Cl2N4O5 (Mr = 539.36): C, 57.89, H, 3.73; N, 10.38; Cl, 13.14%; Found: C, 57.70; H, 3.78; N, 10.29; Cl, 13.09%.Ethyl 4-(4-chlorophenyl)-5-cyano-3-(4-fluorophenyl)-1-(4-nitrophenyl)-2-pyrazoline-5-carboxylate 15d Obtained as yellow crystals; yield: 1.15 g (47%); mp 202-203ºC (from acetic acid); IR (KBr)vmax/cm-1: 3078.7 (CH-aromatic), 2981.4 (CH-aliphatic), 1758.7 (C=O ester), 1589.0 (C=C); 1H NMR (DMSO-d6) δ 7.13-8.42 (m, 12H, ArH's), 6.34 (s, 1H pyrazoline), 4.01 (q, 2H, J 7.1 Hz, COOCH2CH3), 0.90 (t, 3H, J 7.1 Hz, COOCH2CH3); 13C NMR (DMSO-d6) δ 165.17 (d, J 256.1 Hz, C-F), 164.38 (C=O ester), 148.26, 146.25 (C=N, C-p-NO2), 143.43, 133.08, 131.82, 131.29, 129.20 (d, J 8.3 Hz, C-m-F), 128.48, 127.25 (d, J 3.1 Hz, C-p-F), 124.73, 120.98, 115.97 (d, J 22.2 Hz, C-o-F), 111.03 (C≡N), 70.92 (C-5 pyrazoline), 63.35 (C-4 pyrazoline), 61.31 (OCH2CH3), 12.91 (OCH2CH3); MS, m/z: 493 (M++1, 10.1), 492 (M+, 11.5), 420 (88.8), 419 (100.0), 374 (24.5), 373 (25.9), 217 (9.8), 216 (13.3), 122 (13.1), 76 (24.7), 50 (23.0). Anal. Calc. for C25H18ClFN4O4 (Mr = 492.88): C, 60.91; H, 3.68; N, 11.36; Cl, 7.19%; Found: C, 60.87; H, 3.66; N, 11.41; Cl, 7.23%. Ethyl 4-(4-chlorophenyl)-5-cyano-3-(2,4-dichlorophenyl)-1-(4-nitrophenyl)-2-pyrazoline-5-carboxylate 16d Obtained as yellow crystals; yield: 1.38 g (51%); mp 202-204ºC (from acetic acid); IR (KBr)vmax/cm-1: 3064.3 (CH-aromatic), 2983.2 (CH-aliphatic), 1760.2 (C=O ester), 1652.2 (C=N), 1591.1 (C=C); 1H NMR (DMSO-d6) δ 7.14-8.43 (m, 11H, ArH's), 6.23 (s, 1H pyrazoline), 4.18 (q, 2H, J 7.2 Hz, COOCH2CH3), 0.98 (t, 3H, J 7.2 Hz, COOCH2CH3); 13C NMR (DMSO-d6) δ 163.41 (C=O ester), 148.55, 146.11 (C=N, C-p-NO2), 143.84, 134.05, 133.68, 133.26, 132.48, 131.54, 131.28, 130.37, 129.47, 128.50, 126.88, 124.96, 121.78 (13C, ArC's), 112.88 (C≡N), 70.70 (C-5 pyrazoline), 64.27 (C-4 pyrazoline), 61.22 (OCH2CH3), 12.83 (OCH2CH3); MS, m/z: 546 (M++4, 3.9), 544 (M++2, 12.5), 542 (M+, 10.8), 473 (34.9), 471 (100.0), 469 (98.2), 427 (5.1), 425 (17.0), 423 (18.0), 76 (11.0), 75 (11.3), 63 (8.9), 50 (9.9). Anal. Calc. for C25H17Cl3N4O4 (Mr = 543.78): C, 55.21; H, 3.15; N, 10.30; Cl, 19.55%; Found: C, 55.23; H, 3.21; N, 10.19; Cl, 19.48%. Ethyl 5-cyano-3,4-di-(4-fluorophenyl)-1-(4-nitrophenyl)-2-pyrazoline-5-carboxylate 15e Obtained as yellow crystals; yield: 1.47 g (62%); mp 211-213 ºC (from acetic acid); IR (KBr)vmax/cm-1: 3082.9 (CH-aromatic), 2983.4 (CH-aliphatic), 2993.2 (CH-aliphatic), 1758.0 (C=O ester), 1653.9 (C=N), 1591.7 (C=C); 1H NMR (DMSO-d6) δ 7.14-8.43 (m, 12H, ArH's), 6.36 (s, 1H pyrazoline), 4.03-4.14 (q, 2H, J 7.0 Hz, COOCH2CH3), 0.91 (t, 3H, J 7.0 Hz, COOCH2CH3); 13C NMR (DMSO-d6) δ 165.17 (d, J 256.1 Hz, C-F), 164.38 (C=O ester), 161.90 (d, J 256.3 Hz, C-F), 148.26, 146.25 (C=N, C-p-NO2), 143.43, 130.12 (d, J 8.3 Hz, C-m-F), 129.20 (d, J 8.3 Hz, C-m-F), 126.25 (d, J 3.1 Hz, C-p-F), 124.73, 124.62 (d, J 3.1 Hz, C-p-F), 120.97, 115.97 (d, J 22.2 Hz, C-o-F), 115.64 (d, J 22.0 Hz, C-o-F), 111.02 (C≡N), 70.92 (C-5 pyrazoline), 63.35 (C-4 pyrazoline), 61.31 (OCH2CH3), 12.91 (OCH2CH3); MS, m/z: 476 (M+, 13.7), 403 (100.0), 357 (22.7), 76 (10.2), 75 (7.2), 63 (8.0), 50 (6.0). Anal. Calc. for C25H18F2N4O4 (Mr = 476.43): C, 63.02; H, 3.80; N, 11.76%; Found: C, 62.97; H, 3.85; N, 11.87%. Ethyl 5-cyano-3-(2,4-dichlorophenyl)-4-(4-fluorophenyl)-1-(4-nitrophenyl)-2-pyrazoline-5-carboxylate 16e Obtained as yellow crystals; yield: 1.55 g (59%); mp 210-212 ºC (from dioxane - ethanol); IR (KBr)vmax/cm-1: 3069.3 (CH-aromatic), 2991.2 (CH-aliphatic), 1747.6 (C=O ester), 1595.1 (C=C); 1H NMR (DMSO-d6) dδ 7.11-8.42 (m, 11H, ArH's), 6.36 (s, 1H, 4-H pyrazoline), 4.08-4.19 (q, 2H, J 7.2 Hz, COOCH2CH3), 0.92-0.99 (t, 3H, J 7.2 Hz, COOCH2CH3); 13C NMR (DMSO-d6) δ 163.39 (C=O ester), 161.89 (d, J 256.3 Hz, C-F), 148.58, 146.13 (C=N, C-p-NO2), 143.83, 134.05, 133.26, 132.48, 130.37, 130.05 (d, J 8.3 Hz, C-m-F), 129.47, 126.88, 124.96, 124.53 (d, J 3.2 Hz, C-p-F), 121.78, 115.45 (d, J 22.1 Hz, C-o-F), 112.98 (C≡N), 70.71 (C-5 pyrazoline), 64.25 (C-4 pyrazoline), 61.24 (OCH2CH3), 12.84 (OCH2CH3); MS, m/z: 528 (M++2, 9.7), 526 (M+, 15.4), 455 (68.9), 453 (100.0), 407 (23.5), 107 (10.1), 76 (10.7), 63 (12.1). Anal. Calc. for C25H17Cl2FN4O4 (Mr = 527.33): C, 56.93; H, 3.24; N, 10.62; Cl, 13.44%; Found: C, 56.97; H, 3.25; N, 10.60; Cl, 13.46%. Ethyl 5-cyano-3-(4-fluorophenyl)-1,4-di-(4-nitrophenyl)-2-pyrazoline-5-carboxylate 15f Obtained as yellow crystals; yield: 1.33 g (53%); mp 176-178 ºC (from acetic acid); IR (KBr)vmax/cm-1: 3079.0 (CH-aromatic), 2986.3 (CH-aliphatic), 1764.8 (C=O ester), 1654.5 (C=N), 1596.2 (C=C); 1H NMR (DMSO-d6) δ 7.13-8.43 (m, 12H, ArH's), 6.36 (s, 1H pyrazoline), 4.09 (q, 2H, J 7.2 Hz, COOCH2CH3), 0.90 (t, 3H, J 7.2 Hz, COOCH2CH3); 13C NMR (DMSO-d6) δ 165.17 (d, J 256.1 Hz, C-F), 164.38 (C=O ester), 148.26, 146.25 (C=N, C-p-NO2), 143.43, 129.20 (d, J 8.3 Hz, C-m-F), 127.25 (d, J 3.1 Hz, C-p-F), 124.73, 120.97, 115.97 (d, J 22.2 Hz, C-o-F), 111.04 (C≡N), 70.92 (C-5 pyrazoline), 63.35 (C-4 pyrazoline), 61.31 (OCH2CH3), 12.91 (OCH2CH3); MS, m/z: 503 (M++1, 17.4), 476 (62.3), 430 (100.0), 384 (21.1), 76 (20.1), 63 (12.7), 50 (12.5). Anal. Calc. for C25H18FN5O6 (Mr = 503.43): C, 59.64; H, 3.60; N, 13.91%; Found: C, 59.49; H, 3.64; N, 13.87%. Synthesis of 1-aryl-3-(4-nitrophenyl)chromeno[3,4-c]pyrazol-4(3H)-ones 20 To a mixture of hydrazonoyl bromides 1, 2 (5 mmol) and 3-cynocoumarin 19a or 3-phenylsulphonylcoumarin 19b or 3-bromocoumarine 19c (5 mmol), in dry benzene (50 mL) at room temperature, triethylamine (0.7 mL, 5 mmol) was added. The mixture was refluxed till the hydrazonoyl bromide disappeared (8 h) as indicated by TLC analysis. After cooling to room temperature, the precipitated triethylamine hydrobromide was filtered and the solvent evaporated. Trituration of the residue with a small amount of methanol gave a crude solid. The latter was collected, washed with methanol and dried. Crystallization from suitable solvent gave the corresponding chromenopyrazoles 20a, b in good yield. The prepared compounds together with their physical and spectral data are listed below. 1-(4-Fluorophenyl)-3-(4-nitrophenyl)chromeno[3,4-c]pyrazol-4(3H)-one 20a Obtained as brown solid; yield: 0.96 g (48%); mp 310-312ºC (from dioxane); IR (KBr)vmax/cm-1: 3089.6 (CH-aromatic), 1743.4 (C=O, lactone), 1657.8 (C=N), 1605.0 (C=C); 1H NMR (DMSO-d6) δ 7.32-8.45 (12H, ArH's); 13C NMR (DMSO-d6) δ 164.32 (d, J 256.4 Hz, C-F), 156.16 (C=O lactone), 154.31 (C-O), 147.51, 146.24 (C=N, C-p-NO2), 142.40, 132.91, 131.29, 129.24 (d, J 8.3 Hz, C-m-F), 128.92, 126.81, 125.58 (d, J 3.1 Hz, C-p-F), 125.17, 124.24, 121.62, 116.12 (d, J 22.2 Hz, C-o-F), 115.91, 112.15; MS, m/z: 401 (M+, 100.0), 355 (5.9), 257 (49.2), 211 (11.6), 187 (11.7), 163 (18.1), 136 (26.8), 123 (25.5), 90 (52.7), 76 (16.7), 75 (22.1), 63 (43.5), 50 (16.4). Anal. Calc. for C22H12FN3O4 (Mr = 401.34): C, 65.83; H, 3.01; N, 10.47%; Found: C, 65.81; H, 3.00; N, 10.49%.1-(2,4-Dichlorophenyl)-3-(4-nitrophenyl)chromeno[3,4-c]pyrazol-4(3H)-one 20b Obtained as brown solid; yield: 1.15 g (51%); mp 232-234 ºC (from acetic acid); IR (KBr)vmax/cm-1: 3085.3 (CH-aromatic), 1744.2 (C=O lactone), 1654.8 (C=N), 1592.1 (C=C); 1H NMR (DMSO-d6) δ 7.32-8.45 (11H, ArH's); 13C NMR (DMSO-d6) δ 156.16 (C=O lactone), 154.31 (C-O), 147.51, 146.24 (C=N, C-p-NO2), 142.40, 133.63, 132.91, 131.54, 131.29, 129.51, 129.49, 128.92, 128.55, 128.42, 126.81, 125.17, 124.24, 121.62, 115.91, 112.17 (16C, ArC's); MS, m/z: 453 (M++2, 61.0), 451 (M+, 100.0), 206 (11.3), 177 (14.5), 76 (46.7), 75 (49.9), 63 (19.6), 62 (21.5), 51 (13.8), 50 (48.3). Anal. Calc. for C22H11Cl2N3O4 (Mr = 452.24): C, 58.42; H, 2.45; N, 9.29; Cl, 15.67%; Found: C, 58.17; H, 2.47; N, 9.39; Cl, 15.65%.Synthesis of 3a-acetyl(benzoyl)-1-aryl-3-(4-nitrophenyl)-3a,9b-dihydrochromeno[3,4-c]-pyrazol-4(3H)-ones 24, 25 This reaction was carried out by the same method described for the reaction of hydrazonoyl bromides 1, 2 with 3-cyanocoumarin 19a using 3-acetylcoumarin 22 and 3-benzoylcoumarin 23 in place of 3-cyanocoumarin 19a. Crystallization from the suitable solvent gave the corresponding dihydrochromeno[3,4-c]pyrazole derivatives 24a,b and 25a,b respectively in good yield. The prepared compounds together with their physical and spectral data are listed below. 3a-Acetyl-1-(4-fluorophenyl)-3-(4-nitrophenyl)-3a,9b-dihydrochromeno[3,4-c]-pyrazol-4(3H)-one 24a Obtained as off white solid; yield: 1.13 g (51%); mp 288-290 ºC (from dimethylformamide-ethanol); IR (KBr)vmax/cm-1: 3107.7 (CH-aromatic), 2985.6 (CH-aliphatic), 1734.8 (C=O lactone), 1712.9 (C=O acetyl), 1595.0 (C=C); 1H NMR (DMSO-d6) δ 7.14-8.31 (m, 11H, ArH's), 6.07 (s, 1H), 2.57 (s, 3H, CH3 acetyl); 13C NMR (DMSO) δ 201.55 (C=O acetyl), 164.18 (d, J 256.1 Hz, C-F), 163.85 (C=O lactone), 150.87 (C-O), 149.34, 148.66 (C=N, C-p-NO2), 140.70, 130.17, 130.84 (d, J 8.3 Hz, C-m-F), 127.11, 125.21, 125.00, 124.57 (d, J 3.1 Hz, C-p-F), 116.90, 115.54 (d, J 22.2 Hz, C-o-F), 115.96, 112.80, 78.27 (C-3 coumarin), 54.83 (C-4 coumarin), 25.10 (CH3 acetyl); MS, m/z: 427 (M+-18), 401 (100.0), 354 (14.1), 206 (11.5), 76 (30.2), 75 (21.8), 63 (13.0), 50 (20.6). Anal. Calc. for C24H16FN3O5 (Mr = 445.39): C, 64.71; H, 3.62; N, 9.43%; Found: C, 64.70; H, 3.64; N, 9.42%. 3a-Acetyl-1-(2,4-dichlorophenyl)-3-(4-nitrophenyl)-3a,9b-dihydrochromeno[3,4-c]-pyrazol-4(3H)-one 24b Obtained as yellow solid; yield: 1.31 g (53%); mp 196-197 ºC (from acetic acid); IR (KBr) vmax/cm-1: 3107.7 (CH-aromatic), 2989.8 (CH-aliphatic), 1778.0 (C=O), 1712.6 (C=O acetyl), 1590.9 (C=C); 1H NMR (DMSO-d6) δ 7.12-8.27 (m, 11H, ArH's), 6.09 (s, 1H, 4-H coumarin), 2.57 (s, 3H, CH3 acetyl); 13C NMR (DMSO) δ 201.53 (C=O acetyl), 163.48 (C=O lactone), 150.68 (C-O), 149.39, 148.09 (C=N, C-p-NO2), 140.64, 135.71, 133.54, 132.48, 130.64, 129.72, 129.40, 127.72, 127.24, 125.41, 125.30, 116.86, 114.41, 112.52 (14C, ArC's), 79.19 (C-3 coumarin), 54.84 (C-4 coumarin), 25.02 (CH3 acetyl); MS, m/z: 497 (M++2, 4.2), 495 (M+, 6.7), 454 (66.6), 453 (83.1), 452 (83.3), 451 (100.0), 409 (10.7), 408 (15.4), 407 (9.9), 406 (11.8), 373 (17.4), 178 (12.4), 76 (32.7), 50 (30.4). Anal. Calc. for C24H15Cl2N3O5 (Mr = 496.29): C, 58.08; H, 3.04; N, 8.46; Cl, 14.28%; Found: C, 57.72; H, 3.06; N, 8.58; Cl, 14.34%.3a-Benzoyl-1-(4-fluorophenyl)-3-(4-nitrophenyl)-3a,9b-dihydrochromeno[3,4-c]-pyrazol-4(3H)-one 25a Obtained as pale yellow solid; yield: 1.21 g (48%); mp 271-273 ºC (from dioxane); IR (KBr)vmax/cm-1: 3089.4 (CH-aromatic), 1735.3 (C=O), 1655.8 (CO benzoyl), 1594.5 (C=C); 1H NMR (DMSO-d6) δ 7.15-8.13 (m, 17H, ArH's), 6.38 (s, 1H, 4-H coumarin); 13C NMR (DMSO) δ 197.55 (C=O benzoyl), 164.18 (d, J 256.1 Hz, C-F), 162.85 (C=O lactone), 150.92 (C-O), 149.23, 148.68 (C=N, C-p-NO2), 140.70, 132.63, 131.87, 131.31, 130.17, 130.84 (d, J 8.3 Hz, C-m-F), 127.73, 127.10, 125.18, 125.02, 124.57 (d, J 3.1 Hz, C-p-F), 116.90, 116.27 (d, J 22.2 Hz, C-o-F), 115.96, 112.87, 78.27 (C-3 coumarin), 54.83 (C-4 coumarin); MS, m/z: 507 (M+, 53.0), 401 (11.9), 257 (100.0), 211 (12.6), 163 (20.0), 136 (28.2), 123 (66.7), 105 (20.9), 90 (39.3), 77 (34.7), 63 (25.1), 51 (16.1), 50 (13.4). Anal. Calc. for C29H18FN3O5 (Mr = 507.46): C, 68.63; H, 3.57; N, 8.28%; Found: C, 68.60; H, 3.56; N, 8.27%.3a-Benzoyl-1-(2,4-dichlorophenyl)-3-(4-nitrophenyl)-3a,9b-dihydrochromeno[3,4-c]-pyrazol-4(3H)-one 25b Obtained as yellow solid; yield: 1.31 g (47%); mp 193-195ºC (from dioxane); IR (KBr)vmax/cm-1: 3072.0 (CH-aromatic), 1769.3 (br., C=O lactone, benzoyl), 1671 (C=N), 1586.1 (C=C); 1H NMR (DMSO-d6) d 7.13-8.24 (m, 16H, ArH's), 6.36 (s, 1H, 4-H coumarin); 13C NMR (DMSO) d 197.53 (C=O benzoyl), 162.85 (C=O lactone), 150.52 (C-O), 149.20, 148.13 (C=N, C-p-NO2), 140.72, 135.69, 133.31, 132.91, 132.66, 131.76, 131.30, 130.81, 129.76, 129.68, 127.96, 127.70, 126.87, 125.32, 125.12, 116.93, 115.82, 112.73 (18C, ArC's), 78.27 (C-3 coumarin), 54.83 (C-4 coumarin); MS, m/z: 559 (M++2, 3.2), 557 (M+, 10.1), 453 (66.2), 451 (100.0), 405 (11.7), 307 (8.3), 206 (15.5), 178 (18.0), 177 (19.1), 151 (18.9), 105 (30.1), 76 (75.4), 75 (83.8), 63 (31.2), 51 (31.9), 50 (79.1). Anal. Calc. for C29H17Cl2N3O5 (Mr = 558.36): C, 62.37; H, 3.06; N, 7.52; Cl, 12.69%; Found: C, 62.38; H, 3.06; N, 7.51; Cl, 12.68%.Oxidation of chromeno[3,4-c]pyrazol-4(3H)-one derivatives 24 and 25 General procedure A suspension of the products 24a or 25a (5 mmol) in aqueous potassium hydroxide (10 mL, 10%) was refluxed for 12 h. The reaction mixture was cooled, poured into water (50 mL) and acidified with hydrochloric acid (4 mL, mol L-1). The crude product was filtered, washed with water, dissolved in toluene (10 mL) and refluxed for 2 h. After cooling, the product that precipitated was collected and crystallized from suitable solvent. The pure products were identical in all aspects (mp, mixed mp and spectroscopic data) with 20a. Similarly, oxidation of 24b or 25b gave compound 20b.   Results and Discussion 1,3-Dipolar cycloaddition of nitrilimines 3, 4, prepared in situ from hydrazonyl bromides 1, 2 in dry benzene in the presence of triethylamine, to α-cyanocinnamonitriles 5a-f was carried out at reflux for 8 h,24 and gave exclusively 3,4-diaryl-1-(4-nitrophenyl)pyrazole-5-carbonitriles 8a-e, 9a-f respectively (Scheme 1). The intermediate pyrazolines 6, 7 were not detected in any case. The structures of the isolated products 8, 9 were elucidated by their elemental analyses and spectroscopic data. The 1H NMR revealed, in each case, the absence of signals assignable to the 4-CH and 5-CH protons of the corresponding pyrazoline derivatives 6, 7 and 10, 1132 and the IR spectra of the products 8, 9 show a nitrile absorption band at about 2224-2235 cm-1. This finding suggests that the 5,5-dicyano-2-pyrazoline derivatives 6, 7 are easily aromatized by thermal elimination of hydrogen cyanide to give 8, 9. Such elimination is analogous to the thermal elimination of hydrazoic acid33 from 5-azido-5-benzoyl-1,3,4-triphenyl-2-pyrazolin and of benzenesulfinic acid32 from 5-benzenesulfonyl-1,3,4-triphenyl-2-pyrazoline. The signals of 13C NMR spectra of 8b and 9c are compatible with the proposed structure. Thus, they display a signal of methyl carbon at 20.39 and of methoxy carbon at 54.75 ppm. The C≡N signals appear at 110.58 and 110.78 ppm respectively. The regiochemistry of 8, 9 was confirmed by comparison of the properties of 8a with the pertinent regioisomer 3-(4-fluorophenyl)-1-(4-nitrophenyl)-5-phenylpyrazole-4-carbonitrile 7, which was prepared from the reaction of 1 with phenacyl cyanide.34 Similarly, the reaction of 1, 2 with α-cyanocinnamamide 12a, b in benzene at reflux temperature gave the corresponding pyrazole derivatives 13a, b (Scheme 1). Like the previous reaction, thermal elimination of hydrogen cyanide took place. The structures of the products were confirmed considering the correct elemental analyses and spectroscopic data (Experimental part). The 1H NMR spectra of the products 13 showed no signal due to 4-CH characteristic of 2-pyrazoline derivative (Experimental part). This result confirms the elimination of hydrogen cyanide.The reaction of the ethyl α-cyanocinnamates 14a-f with the 1, 2 when carried out in a similar manner, gave the corresponding 2-pyrazoline derivatives 15, 16 (Scheme 2). The thermal elimination of hydrogen cyanide from the reaction products 15, 16 was not observed. The structure of the products was in agreement with their elemental analyses and spectroscopic data. Although compounds 15, 16 bear a nitrile group its IR absorption band does not appear similar to the

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