Quantitative HPLC analysis of sesquiterpene lactones and determination of chemotypes in Eremanthus seidelii MacLeish & Schumacher (Asteraceae)

Journal of the Brazilian Chemical Society

Gobbo-Neto, Leonardo¹  Lopes, João L. C.¹  Universidade de São Paulo, Ribeirão Preto, Brazil¹  Lopes, Norberto P.¹  Sakamoto, Humberto T.¹  Cavalheiro, Alberto J.¹  Universidade Estadual Paulista, Araraquara, Brazil¹ 

关键词: HPLC;    sesquiterpene lactones;    chemotypes;    populational variation;    Eremanthus seidelii;   

DOI  :  10.1590/S0103-50532005000800016

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

Eremanthus seidelii MacLeish & Schumacher has a restricted occurrence to the Brazilian "cerrado" surrounding the Furnas (MG) reservoir, in environments that have been seriously damaged by human activity. The present phytochemical investigation reveals that the sesquiterpene lactones (SL) 4b,5-dihydro-2',3'-dihydroxy-15-desoxy-goyazensolide (1) and 4b,5-dihydro-1',2'-epoxy-eremantholide-C (2) are the major secondary metabolites in E. seidelii leaves, and an HPLC method was developed for their quantitative analysis. HPLC analysis showed no significant seasonal variation in the concentrations of both SL. No qualitative differences were found in the SL patterns of all individuals sampled. However, there is a different SL quantitative pattern among the plants analyzed, pointing to the existence of three quantitative chemotypes of this species, with differences possibly originating from the activity of the enzymes that cyclize the goyazensolide type SL (1) to a eremantholide type SL (2).

【 授权许可】

Unknown