Synthesis and bio-catalytic activity of isostructural cobalt(III)-phenanthroline complexes

Journal of Chemical Sciences

Anandan Ranjani¹  Hui-Lien Tsai²  Bhaskar Biswas1⁵  Mohammad Abdulkader Akbarsha³  Loganathan Gayathri¹  Milan Maji⁴  Arnab Basu Roy⁵  Dharumadurai Dhanasekaran¹  Niranjan Kole⁵  Chung-Yu Shen²  Dhananjay Dey⁵  Saravanan Chandraleka2¹ 

[1] Department of Microbiology, Bharathidasan University, Tiruchirappalli 620 024, India$$;Department of Chemistry, National Cheng Kung University, Tainan City, 70101, Taiwan$$;Mahatma Gandhi-Doerenkamp Centre, Bharathidasan University, Tiruchirappalli 620 024, India$$;Department of Chemistry, National Institute of Technology, Mahatma Gandhi Avenue, Durgapur 713209, India$$;Department of Chemistry, Raghunathpur College, Purulia 723133, India$$

关键词: Cobalt(III);    X-ray structure;    Catecholase activity;    DNA cleavage;    Anti-cancer activity.;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

We have synthesized two isostructural mononuclear cobalt(III) complexes [1]NO3·3H2O and [1]NO3·CH3CO2H·H2O {[1]+ = [Co(1,10-phenanthroline)2Cl2]+} and characterized by single crystal X-ray structural analyses. Mass spectral studies of the complexes indicate both the compounds to produce identical cationic species viz., [Co(phen)2Cl2]+ in methanol solution. [1]+ has been evaluated as model system for the catechol oxidase enzyme by using 3,5-di-tert-butylcatechol (3,5-DTBC) as the substrate in methanol medium, which revealed that the cationic complex efficiently inhibits catalytic activity with kcat value 9.65 × 102 h−1. [1]+ cleaved pBR 322 DNA without addition of an activating agent. Further, the anti-cancer activity of [1]+ on human hepatocarcinoma cell line (HepG2) has been examined. The induction of apoptosis induced in the cell line was assessed base on the changes in cell morphology, which showed the efficacy of [1]+ to induce apoptosis in 53% of cells during 24 h treatment. Interestingly, the observed IC50 values reveal that [1]+ brings about conformational change on DNA strongly and exhibits remarkable cytotoxicity.

【 授权许可】

Unknown