Journal of Chemical Sciences | |
Biocatalysis by metallated cyclotriphosphazenes: L2Zn(NO3)2 {L = spiro-N3P3[O2C12H8][N(CH3)NH2]} as a synthetic phosphoesterase and nuclease | |
C Madhavaiah1  Ramachandran Azhakar1  Sandeep Verma1  Vadapalli Chandrasekhar11  Venkatasubbiah Krishnan1  | |
[1] Department of Chemistry, Indian Institute of Technology, Kanpur 208 016, India$$ | |
关键词: Cyclophosphazene hydrazides; metalated cyclophosphazenes; zinc complexes; phosphate ester hydrolysis; DNA cleavage.; | |
DOI : | |
来源: Indian Academy of Sciences | |
【 摘 要 】
Catalytic activity of [L2.Zn][NO3]2 (L = spiro-N3P3[O2C12H8][N(CH3)NH2]) towards the hydrolysis of two phosphodiesters, [bis(ð‘-nitrophenyl)phosphate, bNPP] and [2-(hydroxypropyl)-p-nitrophenyl phosphate, hNPP] has been examined. While the rate of hydrolysis of the former is accelerated over a million-fold, the rate of hydrolysis of the latter also is enhanced considerably. Detailed kinetic evaluation of these reactions has been carried out and all the kinetic parameters including the Michaelis-Menten parameters are reported. The catalyst [L2.Zn][NO3]2 has also been found to be an effective nuclease. Relaxation of supercoiled plasmid DNA, pBR322, occurs in presence of [L2.Zn][NO3]2 without the need for any exogenous reagents.
【 授权许可】
Unknown
【 预 览 】
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RO201912040507674ZK.pdf | 75KB | download |