Radix ranunculus temate saponins induces apoptosis via the death receptor and mitochondrial pathways in SGC-7901 cells

Molecular & Cellular Toxicology

Qian-na Gu¹  Hong-ya Hang¹  Wen-yan Wu¹  Yao-li Jiang¹  Jiao Jiang¹  Mao-lin Li¹  Han-min Gu¹ 

[1] Yixing Tumor Hospital$$

关键词: Mitochondrial pathway;   

DOI  :  10.1007/s13273-015-0048-9

学科分类：分子生物学，细胞生物学和基因

来源: Korean Society of Toxicogenomics and Toxicoproteomics

【 摘 要 】

adix ranunculus temate saponins (RRTS), one of the main constituents extracted from the popular traditional Chinese medicine Radix Ranunculi ternati, has been reported to have various biological activities including anti-cancer effect. The aim of this study is to investigate the effect of RRTS on the cell proliferation and apoptosis in human gastric adenocarcinoma SGC-7901 cells. The data showed that exposure to RRTS for 24 h produced cytotoxic effects on SGC- 7901 cells in a dose-dependent manner (with an IC₅₀ value of 21.22±2.76 μg/mL), which was accompanied by apoptosis induction (from 2.18±0.89% (control) to 63.72±13.16% (100 μg/mL)). Both the extrinsic or death receptor pathway and the intrinsic or mitochondrial pathway were involved in RRTS-induced apoptosis in SGC-7901 cells. Furthermore, apoptotic signaling induced by RRTS was amplified by cross-link between the two pathways via the signal-integrating protein Bid. In conclusion, our findings contribute to better understanding the molecular mechanism of RRTS�? effect on gastric cancer cells and form the basis of the therapeutic development of RRTS in treating gastric cancer in the future.

【 授权许可】

Unknown