Changes of AXIN-1 and Beta-Catenin in Neuroepithelial Brain Tumors

Pathology & Oncology Research

Vesna Kušec⁴  Davor Tomas²  Hana Mušinović³  Martina Zeljko³  Nives Pećina-Šlaus¹  Tomislav Kokotović³  Tamara Nikuševa Martić¹ 

[1] School of Medicine University of Zagreb$$University of Zagreb$$;University Hospital “Sisters of Charity�?$$;School of Medicine University of Zagreb$$;Clinical Hospital Centre Zagreb$$

关键词: Wnt signaling;   

DOI  :  10.1007/s12253-009-9190-9

学科分类：生理学与病理学

来源: Springer

【 摘 要 】

In the present study changes of components of Wnt signaling pathway—axin (AXIN1) and beta-catenin (CTNNB1) in a sample of 72 neuroepithelial brain tumors were investigated. AXIN-1 gene was tested by PCR/loss of heterozygosity (LOH). Immunostaining and image analysis revealed the quantity and localization of relevant proteins. Polymorphic marker for AXIN-1, showed LOH in 11.1% of tumors. LOH was distributed to 6.3% of glioblastomas, one was found in neuroepithelial dysembrioplastic tumor and one in medulloblastoma. Down regulation of axin expression and up regulation of beta-catenin were detected in the analyzed tumors. Axin was observed in the cytoplasm in 68.8% of samples, in 28.1% in both the cytoplasm and nucleus and 3.1% had no expression. Beta-catenin was observed mainly in the nucleus and cytoplasm (59.4%). Expression in 34.4% of samples was in the cytoplasm and 6.3% showed no expression. Comparison of mean values of relative increase of axin and beta-catenin showed that they are significantly reversely proportional (P�?=�?0.014). Relative quantity of beta-catenin in patients with gross deletion of AXIN1 was significantly higher in comparison to patients without LOH (P�?=�?0.040). Our results demonstrate that changes of key components of the Wnt signaling play a role in neuroepithelial brain tumors.

【 授权许可】

Unknown