Direct Flight Muscles in Drosophila Develop from Cells with Characteristics of Founders and Depend on DWnt-2 for Their Correct Patterning

Developmental Biology

Karen M. Kozopas¹  Roel Nusse¹ 

[1] Department of Cell Biology, University of Alabama at Birmingham, Birmingham, Alabama, 35294-0005

关键词: muscle development;    Wnt signaling;    epithelial attachments;    direct flight muscles;   

DOI  :  10.1006/dbio.2002.0572

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Thedirectflightmuscles(DFMs)ofDrosophilaallowforthefinecontrolofwingpositionnecessaryforflight.InDWnt-2mutantflies,certainDFMsareeithermissingorfailtoattachtothecorrectepithelialsites.Usingatemperature-sensitiveallele,weshowthatDWnt-2activityisrequiredonlyduringpupationforcorrectDFMpatterning.DWnt-2isexpressedintheepitheliumofthewinghingeprimordiumduringpupation.ThisexpressionisinthevicinityofthedevelopingDFMs,asrevealedbyexpressionofthemusclefoundercell-specificgenedumbfoundedinDFMprecursors.TheobservationthatagenenecessaryforembryonicfoundercellfunctionisexpressedintheDFMprecursorssuggeststhatthesecellsmayhaveasimilarfoundercellrole.AlthoughtheexpressionpatternofDWnt-2suggeststhatitcouldinfluenceepithelialcellstodifferentiateintoattachmentsitesformuscle,theexpressionofstripe,atranscriptionfactornecessaryforepithelialcellstoadoptanattachmentcellfate,isunalteredinthemutant.EctopicexpressionofDWnt-2inthewinghingeduringpupationcanalsocreatedefectsinmusclepatterningwithoutalterationsinstripeexpression.WeconcludethatDWnt-2promotesthecorrectpatterningofDFMsthroughamechanismthatisindependentoftheattachmentsitedifferentiationinitiatedbystripe.

【 授权许可】

Unknown