Immunohistochemical assessment and prognostic value of hepatitis B virus X protein in chronic hepatitis and primary hepatocellular carcinomas usinganti-HBxAg monoclonal antibody

Pathology & Oncology Research

Péter Németh³  János Molnár¹  Csilla Somogyi³  Ágnes Szmolenszky³  József Sípos⁴  György Szekeres²  József Pál³  Géza Hegedüs⁵  Ilona Martzinovits¹ 

[1] University of Szeged$$;Histopatology Ltd.$$;University of Pécs, Faculty of Medicine$$;County Hospital of Zala$$;County Hospital of Baranya$$

关键词: immunohistochemistry;   

DOI  :  10.1007/BF03032346

学科分类：生理学与病理学

来源: Springer

【 摘 要 】

Hepatitis B virus (HBV) is the most meaningful risk factor in chronic hepatitis, cirrhosis and primary hepatocellular carcinoma (PHC). The hepatitis B virus X protein (HBxAg) is a multifunctional protein with many important functions in hepatocellular carcinogenesis. A monoclonal anti-HBxAg antibody was developed in our laboratory and characterized by different methods. Using this antibody HBxAg was detected in formaldehyde fixed paraffin embedded tissue sections of 72 liver biopsies from patients with acute hepatitis, chronic hepatitis, cirrhosis and primary hepatocellular carcinoma. The co-expression of hepatitis B surface antigen (HBsAg), hepatitis B core antigen (HBcAg) and HBxAg was compared. The histological and cytological localization of the detected HBxAg showed a characteristic distribution in different stages of HBV infection. Strong and diffuse nuclear reaction was detected in PHC cases in contrast to the focal, cytoplasmic and nuclear labeling in the acute and chronic B hepatitis cases. Our antibody seems to be a suitable prognostic marker for routine pathohistological diagnosis and for comparative pathological and epidemiological research on the development of PHC.

【 授权许可】

Unknown