Cordyceps bassiana inhibits smooth muscle cell proliferation via the ERK1/2 MAPK signaling pathway

Cellular & Molecular Biology Letters

Seongho Han³  Mina Son¹  Sung-Whan Kim²  Enze Jin⁴ 

[1] Department of Medicine, College of Medicine, Catholic Kwandong University, Gangneung, Republic of Korea$$;Department of Medicine, College of Medicine, Catholic Kwandong University, Gangneung, Republic of Korea$$International St. Mary’s Hospital, Incheon, Republic of Korea$$;Department of Family Medicine, College of Medicine, Dong-A University, Busan, Republic of Korea$$;Department of Cardiology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, China$$

关键词: Cordyceps;    ERK pathway;    Inhibitory;    MAPK;    Mechanism;    Natural product;    Neointimal formation;    Proliferation;    Smooth muscle cells;    Vascular;   

DOI  :  10.1186/s11658-016-0023-z

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

Cordyceps belongs to a genus of acormycete fungi and is known to exhibit various pharmacological effects. The aim of this study was to investigate the effect of Cordyceps species on the proliferation of vascular smooth muscle cells (VSMC) and their underlying molecular mechanism. A cell proliferation assay showed that Cordyceps bassiana ethanol extract (CBEE) significantly inhibited VSMC proliferation. In addition, neointimal formation was significantly reduced by treatment with CBEE in the carotid artery of balloon-injured rats. We also investigated the effects of CBEE on the extracellular signal-regulated kinase (ERK) signal pathway. Western blot analysis revealed increased ERK 1/2 phosphorylation in VSMCs treated with CBEE. Pretreatment with U0126 completely abrogated CBEE-induced ERK 1/2 phosphorylation. In conclusion, CBEE exhibited anti-proliferative properties that affected VSMCs through the ERK1/2 MAPK signaling pathway. Our data may elucidate the inhibitory mechanism of this natural product.

【 授权许可】

Unknown