IgG silencing induces apoptosis and suppresses proliferation, migration and invasion in LNCaP prostate cancer cells

Cellular & Molecular Biology Letters

Yawen Xu¹  Abai Xu¹  Yong Wen¹  Bingkun Li¹  Binshen Chen¹  Kai Xu¹  Shaobo Zheng¹  Chunxiao Liu¹ 

[1] Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, China$$

关键词: LNCaP cells;    Prostate cancer;    RNA interference;    Immunoglobulin G;    Proliferation;    Apoptosis;    Migration;    Invasion;    Cell cycle;    Caspase-3;   

DOI  :  10.1186/s11658-016-0029-6

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

Immunoglobulin G (IgG) has been implicated in the progression of various cancers. This study explored the role of IgG in the proliferation, apoptosis, cell cycle and in vitro invasive properties of LNCaP prostate cancer cells. We used IGHG1 small interfering RNA to silence IgG1 expression in LNCaP cells. The efficacy of IgG1 gene knockdown was confirmed using qPCR and western blotting. The colony formation, proliferation, migration and invasion abilities of LNCaP cells after transfection were assessed using colony-forming, flow cytometry and transwell assays. The expressions of PCNA and caspase-3 proteins in LNCaP cells after transfection were detected with immunofluorescence staining and western blotting. IgG1 silencing significantly decreased the colony formation, survival, cell cycle progression, migration and invasion of LNCaP cells (p < 0.05). IgG1 silencing also reduced the amount of the proliferation marker PCNA and induced formation of the apoptotic marker caspase-3 (p < 0.05). Our results show that IgG1 produced by LNCaP cells confers advantages for tumor cell survival, proliferation, migration and invasion, suggesting that IgG1 is a potential target for prostate cancer treatment.

【 授权许可】

Unknown