MiR-34a regulates apoptosis in liver cells by targeting the KLF4 gene

Cellular & Molecular Biology Letters

Jun Hou¹  Lu Lin Zheng³  Xue Yong Zuo³  Lei Li³  Yong Ming He²  Qiu Chen³  Shu Yu Zhang³  Jian Ping Cao³  Feng Mei Cui³  Wei Liu³  Wei Zhu³  Yu Tu³ 

[1]Department of Pathology, Zhongshan Hospital, Fudan University, Shanghai City, China$$

[2]Department of Cardiology, the First Affiliated Hospital of Soochow University, Suzhou City, China$$

[3]Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou City, China$$

关键词: MiR-34a;    KLF4;    Apoptosis;    Liver;    Irradiation;    Doxorubicin;   

DOI  :  10.2478/s11658-013-0115-y

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

MicroRNAs (miRNAs) regulate gene expression by inhibiting translation or targeting messenger RNA (mRNA) for degradation in a posttranscriptional fashion. In this study, we show that ectopic expression of miR-34a-5p reduces the mRNA and protein levels of Krüppel-like factor 4 (KLF4). We also demonstrate that miR-34a targets the 3′-untranslated mRNA region of KLF4 and show that overexpression of miR-34a induces a significant level of apoptosis in BNL CL.2 cells exposed to doxorubicin or 10 Gy X-ray. Our data suggest that the effects of miR-34a on apoptosis occur due to the downregulation of KLF4.

【 授权许可】

Unknown