Autophagy favors Brucella melitensis survival in infected macrophages

Cellular & Molecular Biology Letters

Chuangfu Chen¹  Shengwei Hu²  Yuanzhi Wang³  Ke Zhang¹  Jun Qiao¹  Yong Wang¹  Guoqing Du¹  Fei Guo¹  Hui Zhang²  Yan Ren³ 

[1] College of Animal Science and Technology, Shihezi University, Shihezi, China$$;Key Laboratory of Xijiang Endemic and Ethnic Disease, Shihezi University, Shihezi, China$$;College of Medicine, Shihezi University, Shihezi, China$$

关键词: Autophagy;    LC3-II;    LC3-I;    Autophagosome;    Autophagy flux;    3-Methyladenine;    Intracellular pathogens;    Murine macrophage RAW264.7;    Autophagic vesicle;   

DOI  :  10.2478/s11658-012-0009-4

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

This study investigated the role of autophagy in the survival of the invasive bacterium Brucella melitensis strain 16M in murine macrophages. Here, Brucella melitensis 16M was found to trigger autophagosome formation, enhance autophagy flux and increase the expression level of the autophagy marker protein LC3-II. When autophagy was pharmacologically inhibited by 3-methyladenine (3-MA), Brucella replication efficiency was significantly decreased (p < 0.05). These results suggest that autophagy favors Brucella melitensis 16M survival in murine macrophages.

【 授权许可】

Unknown