Developmental expression of P 5 ATPase mRNA in the mouse

Cellular & Molecular Biology Letters

Lisa S. Weingarten³  Hardi Dave¹  Dorota A. Crawford²  Hongyan Li¹ 

[1] School of Kinesiology and Health Science, Faculty of Health, York University, Toronto, Canada$$;Department of Biology, York University, Faculty of Science and Engineering, Toronto, Canada$$School of Kinesiology and Health Science, Faculty of Health, York University, Toronto, Canada$$Neuroscience Graduate Diploma Program, Faculty of Health, York University, Toronto, Canada$$;Department of Biology, York University, Faculty of Science and Engineering, Toronto, Canada$$

关键词: mRNA expression;    Neurogenesis;    Parkinson’s disease;    Autism spectrum disorders;    Real-time PCR;    Immunohistochemistry;   

DOI  :  10.2478/s11658-011-0039-3

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

P5 ATPases (ATP13A1 through ATP13A5) are found in all eukaryotes. They are currently poorly characterized and have unknown substrate specificity. Recent evidence has linked two P5 ATPases to diseases of the nervous system, suggesting possible importance of these proteins within the nervous system. In this study we determined the relative expression of mouse P5 ATPases in development using quantitative real time PCR. We have shown that ATP13A1 and ATP13A2 were both expressed similarly during development, with the highest expression levels at the peak of neurogenesis. ATP13A3 was expressed highly during organogenesis with one of its isoforms playing a more predominant role during the period of neuronal development. ATP13A5 was expressed most highly in the adult mouse brain. We also assessed the expression of these genes in various regions of the adult mouse brain. ATP13A1 to ATP13A4 were expressed differentially in the cerebral cortex, hippocampus, brainstem and cerebellum while levels of ATP13A5 were fairly constant between these brain regions. Moreover, we demonstrated expression of the ATP13A4 protein in the corresponding brain regions using immunohistochemistry. In summary, this study furthers our knowledge of P5-type ATPases and their potentially important role in the nervous system.

【 授权许可】

Unknown