Identification of molecules derived from human fibroblast feeder cells that support the proliferation of human embryonic stem cells

Cellular & Molecular Biology Letters

Jia-Yi Li²  Ana S. Correia⁴  Patrik Brundin²  Sergey V. Anisimov¹  Ingrid Sandelin³  Vanessa J. Hall⁵  Nicolaj S. Christophersen² 

[1] Neuronal Survival Unit, Wallenberg Neuroscience Center, Department of Experimental Medical Science, Lund University, Lund, Sweden$$Research Department of Cell and Gene Engineering, V. A. Almazov Federal Center for Heart, Saint-Petersburg, Russia$$Department of Intracellular Signalling and Transport, Institute of Cytology, Russian Academy of Sciences, Saint-Petersburg, Russia$$;Neuronal Survival Unit, Wallenberg Neuroscience Center, Department of Experimental Medical Science, Lund University, Lund, Sweden$$;Neuronal Survival Unit, Wallenberg Neuroscience Center, Department of Experimental Medical Science, Lund University, Lund, Sweden$$IVF Kliniken Cura, Malmö, Sweden$$;Neuronal Survival Unit, Wallenberg Neuroscience Center, Department of Experimental Medical Science, Lund University, Lund, Sweden$$Faculty of Medicine, Centre de Recherche du CHUL, Neuroscience Axis, Université Laval, Québec, Canada$$;Neuronal Survival Unit, Wallenberg Neuroscience Center, Department of Experimental Medical Science, Lund University, Lund, Sweden$$Department of Basic Animal and Veterinary Sciences, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark$$

关键词: Human embryonic stem cells;    Feeder cells;    DNA microarray;   

DOI  :  10.2478/s11658-010-0039-8

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

The majority of human embryonic stem cell lines depend on a feeder cell layer for continuous growth in vitro, so that they can remain in an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms that underlie the capacity of feeder cells to support both the proliferation and pluripotency of these cells. Importantly, feeder cells generally lose their capacity to support human embryonic stem cell proliferation in vitro following long-term culture. In this study, we performed large-scale gene expression profiles of human foreskin fibroblasts during early, intermediate and late passages using a custom DNA microarray platform (NeuroStem 2.0 Chip). The microarray data was validated using RT-PCR and virtual SAGE analysis. Our comparative gene expression study identified a limited number of molecular targets potentially involved in the ability of human neonatal foreskin fibroblasts to serve as feeder cells for human embryonic stem cell cultures. Among these, the C-KIT, leptin and pigment epithelium-derived factor (PEDF) genes were the most interesting candidates.

【 授权许可】

Unknown