Flavonoid transport across RBE4 cells: A blood-brain barrier model

Cellular & Molecular Biology Letters

Ana Faria²  Diana Teixeira¹  Nuno Mateus³  Diogo Pestana¹  Victor Freitas³  Conceição Calhau¹  Joana Azevedo³ 

[1] Department of Biochemistry (U38-FCT), Faculty of Medicine of the University of Porto, Porto, Portugal$$;Department of Biochemistry (U38-FCT), Faculty of Medicine of the University of Porto, Porto, Portugal$$Chemistry Investigation Centre (CIQ), Department of Chemistry, Faculty of Sciences, University of Porto, Porto, Portugal$$;Chemistry Investigation Centre (CIQ), Department of Chemistry, Faculty of Sciences, University of Porto, Porto, Portugal$$

关键词: Anthocyanin;    Blood-brain barrier;    Flavonol;    3-flavanol;    RBE4;    Transport;   

DOI  :  10.2478/s11658-010-0006-4

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

There is a growing interest in dietary therapeutic strategies to combat oxidative stress-induced damage to the Central Nervous System (CNS), which is associated with a number of pathophysiological processes, including Alzheimer’s and Parkinson’s diseases and cerebrovascular diseases. Identifying the mechanisms associated with phenolic neuroprotection has been delayed by the lack of information concerning the ability of these compounds to enter the CNS. The aim of this study was to evaluate the transmembrane transport of flavonoids across RBE-4 cells (an immortalized cell line of rat cerebral capillary endothelial cells) and the effect of ethanol on this transport. The detection and quantification of all of the phenolic compounds in the studied samples (basolateral media) was performed using a HPLC-DAD (Diode Array Detector). All of the tested flavonoids (catechin, quercetin and cyanidin-3-glucoside) passed across the RBE-4 cells in a time-dependent manner. This transport was not influenced by the presence of 0.1% ethanol. In conclusion, the tested flavonoids were capable of crossing this blood-brain barrier model.

【 授权许可】

Unknown