The ceramide structure of GM1 ganglioside differently affects its recovery in low-density membrane fractions prepared from HL-60 cells with or without triton-X100

Cellular & Molecular Biology Letters

Tadeusz Pacuszka¹  Grażyna Hoser²  Hanna Domek¹  Natalia Fedoryszak¹  Maciej Kawalec²  Mirosława Panasiewicz¹ 

[1] Departments of Biochemistry and Molecular Biology, Medical Center of Postgraduate Education, Marymoncka 99, Poland$$;Department of Clinical Cytology, Medical Center of Postgraduate Education, Marymoncka 99, Poland$$

关键词: Ceramide;    Gangliosides;    GM1;    Membrane domains;    Myristic acid;    Sonication;   

DOI  :  10.2478/s11658-008-0043-4

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

Gangliosides are characteristically enriched in various membrane domains that can be isolated as low density membrane fraction insoluble in detergents (detergent-resistant membranes, DRMs) or obtained after homogenization and sonication in 0.5 M sodium carbonate (low-density membranes, LDMs). We assessed the effect of the ceramide structure of four [3H]-labeled GM1 ganglioside molecular species (GM1s) taken up by HL-60 cells on their occurrence in LDMs, and compared it with our previous observations for DRMs. All GM1s contained C18 sphingosine, which was acetylated in GM1(18:1/2) or acylated with C14, C18 or C18:1 fatty acids (Fas)

【 授权许可】

Unknown