Cell separation with horizontal cell electrophoresis under near-isopycnic conditions on a “density cushion”

Cellular & Molecular Biology Letters

Anna Wilk²  David E. Woolley¹  Katarzyna Urbańska²  Włodzimierz Korohoda² 

[1] University Department of Medicine, Manchester Royal Infirmary, Manchester, UK$$;Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland$$

关键词: Cell electrophoresis;    Cell separation;    Cell surface;   

DOI  :  10.2478/s11658-008-0007-8

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

This report describes an improvement made to the horizontal cell electrophoresis methodology. It involves using two liquid layers differing in density to produce an interface described as a “density cushion”. The electrophoretic system that employed an anti-convective porous matrix to separate red blood cells (RBC) and charged dyes effectively was found to be unsuitable for some other mammalian cells. The “density cushion” method was found to be more versatile and applicable to studies on the separation of a variety of cell types. The experiments described show the differences between the electrophoretic mobilities of a human eosinophilic leukaemia cell line (Eol-1) and RBC, both with and without the modification of the cell surface properties.

【 授权许可】

Unknown