The induction of apoptosis by daunorubicin and idarubicin in human trisomic and diabetic fibroblasts

Cellular & Molecular Biology Letters

Sylwia Dragojew³  Krzysztof Ilnicki¹  Janusz Maszewski²  Zofia Jóźwiak³  Agnieszka Marczak³ 

[1] Department of Medical Genetics, Institute of the Centrum of Child Health, Warszawa, Poland$$;Department of Cytophysiology, University of Łódź, Łódź, Poland$$;Department of Thermobiology, University of Łódź, Łódź, Poland$$

关键词: Daunorubicin;    Idarubicin;    Apoptosis;    Fibroblasts;    Down’s syndrome;    Diabetes;   

DOI  :  10.2478/s11658-007-0045-7

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

In this study, we investigated apoptosis induced in human trisomic and diabetic fibroblasts by daunorubicin (DNR) and its derivative, idarubicin (IDA). The cells were incubated with DNR or IDA for 2 h and then cultured in a drug-free medium for a further 2–48 h. The apoptosis in the cultured cell lines was assessed by biochemical analysis. We found that both drugs induced a timedependent loss of mitochondrial membrane potential, and a significant increase in intracellular calcium and caspase-3 activity. Mitochondrial polarization and changes in the level of intracellular calcium were observed during the first 2–6 h after drug treatment. Caspase-3 activation occurred in the late stages of the apoptotic pathway. Our findings also demonstrated that idarubicin was more cytotoxic and more effective than daunorubicin in inducing apoptosis in trisomic and diabetic fibroblasts.

【 授权许可】

Unknown