Procaspase-9 is attached to the mitochondrial outer membrane in the early stages of apoptosis

Cellular & Molecular Biology Letters

Dušan Šuput¹  Irina Milisav¹ 

[1]Medical Faculty, Institute of Pathophysiology, University of Ljubljana, Ljubljana, Slovenia$$

关键词: Caspase-9;    Procaspase-9;    Mitochondria;    Apoptosis;    Protein import;    Rotenone;    Localization;   

DOI  :  10.2478/s11658-007-0020-3

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

Procaspase-9 is the zymogen form of one of the apoptosis initiators, caspase-9. Its cellular location may differ depending on the cell type; it is found throughout the cytosol, although some of it may be associated with the mitochondria. Procaspase-9 relocates from the cytosol to the mitochondria shortly after the triggering of apoptosis in rat hepatocytes. We investigated whether the mitochondrial protein import machineries import procaspase-9. The combined results of protein import analyses, mitochondrial fractionation and protease treatments of intact and swollen mitochondria imply that procaspase-9 attaches to the outer surface of the mitochondrial outer membrane.

【 授权许可】

Unknown