Emulsions of oil from Adenanthera pavonina L. seeds and their protective effect

Cellular & Molecular Biology Letters

Anna Jaromin¹  Robert Żarnowski²  Arkadiusz Kozubek¹ 

[1] Department of Lipids and Liposomes, Institute of Biochemistry and Molecular Biology, University of Wrocław, Wrocław, Poland$$;Department of Medicinal Microbiology & Immunology, 424 Medical Science Centre, University of Wisconsin — Madison, Madison, USA$$

关键词: Submicron oil-in-water emulsion;    Hemolysis;    Cardol;   

DOI  :  10.2478/s11658-006-0037-z

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

In our previous study, we developed very stable formulations of submicron oil-in-water emulsions from Adenanthera pavonina L. (family Leguminosae, subfamily Mimosoideae) seed oil, stabilised with soybean lecithin (SPC). Continuing our research, we introduced an additional co-emulsifier, Tween 80, to those formulations in order to decrease the size of the emulsion particles and improve their stability. Formulations with a mean particle size ranging from 43.6 to 306.5 nm and a negative surface charge from −45.3 to −28.5 mV were obtained. Our stability experiments also revealed that most of the tested formulations had a very good degree of stability over a 3-month storage period, both at 4°C and at room temperature. Since many intravenous injectable drugs exhibit lytic activity against erythrocytes, we examined this activity for the emulsion form of cardol, a natural compound with already proven hemolytic properties. The incorporation of this agent into the emulsion caused an evident decrease in hemolytic activity (97–99%). This highly protective effect, observed against sheep erythrocytes, was independent of both the composition and the particle size of the emulsions used. Our studies suggest that nonionic surfactant/phospholipid-based emulsions containing this edible oil of A. pavonina L. may be useful as an alternative formulation matrix for pharmaceutical, nutritional or cosmetic applications of otherwise membrane-acting components.

【 授权许可】

Unknown