期刊论文详细信息
BMC Complementary and Alternative Medicine
In vitro antiproliferative/cytotoxic activity on cancer cell lines of a cardanol and a cardol enriched from Thai Apis mellifera propolis
Research Article
Dungporn Teerasripreecha1  Chanpen Chanchao2  Preecha Phuwapraisirisan3  Masayuki Okuyama4  Atsuo Kimura4  Haruhide Mori4  Kiyoshi Kimura5  Songchan Puthong6 
[1] Department of Biology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, 10330, Bangkok, Thailand;Department of Biology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, 10330, Bangkok, Thailand;Department of Biology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, 10330, Bangkok, Thailand;Department of Chemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, 10330, Bangkok, Thailand;Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, 060-8589, Sapporo, Japan;Honeybee Research Group, National Institute of Livestock and Grassland Science, 305-0901, Ibaraki, Japan;Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, 254 Phayathai Road, 10330, Bangkok, Thailand;
关键词: Antiproliferative activity;    Apis mellifera;    Propolis;    Cancer cell;    Cardanol;    Cardol;   
DOI  :  10.1186/1472-6882-12-27
 received in 2012-01-09, accepted in 2012-03-30,  发布年份 2012
来源: Springer
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【 摘 要 】

BackgroundPropolis is a complex resinous honeybee product. It is reported to display diverse bioactivities, such as antimicrobial, anti-inflammatory and anti-tumor properties, which are mainly due to phenolic compounds, and especially flavonoids. The diversity of bioactive compounds depends on the geography and climate, since these factors affect the floral diversity. Here, Apis mellifera propolis from Nan province, Thailand, was evaluated for potential anti-cancer activity.MethodsPropolis was sequentially extracted with methanol, dichloromethane and hexane and the cytotoxic activity of each crude extract was assayed for antiproliferative/cytotoxic activity in vitro against five human cell lines derived from duet carcinoma (BT474), undifferentiated lung (Chaco), liver hepatoblastoma (Hep-G2), gastric carcinoma (KATO-III) and colon adenocarcinoma (SW620) cancers. The human foreskin fibroblast cell line (Hs27) was used as a non-transformed control. Those crude extracts that displayed antiproliferative/cytotoxic activity were then further fractionated by column chromatography using TLC-pattern and MTT-cytotoxicity bioassay guided selection of the fractions. The chemical structure of each enriched bioactive compound was analyzed by nuclear magnetic resonance and mass spectroscopy.ResultsThe crude hexane and dichloromethane extracts of propolis displayed antiproliferative/cytotoxic activities with IC50 values across the five cancer cell lines ranging from 41.3 to 52.4 μg/ml and from 43.8 to 53.5 μg/ml, respectively. Two main bioactive components were isolated, one cardanol and one cardol, with broadly similar in vitro antiproliferation/cytotoxicity IC50 values across the five cancer cell lines and the control Hs27 cell line, ranging from 10.8 to 29.3 μg/ml for the cardanol and < 3.13 to 5.97 μg/ml (6.82 - 13.0 μM) for the cardol. Moreover, both compounds induced cytotoxicity and cell death without DNA fragmentation in the cancer cells, but only an antiproliferation response in the control Hs27 cells However, these two compounds did not account for the net antiproliferation/cytotoxic activity of the crude extracts suggesting the existence of other potent compounds or synergistic interactions in the propolis extracts.ConclusionThis is the first report that Thai A. mellifera propolis contains at least two potentially new compounds (a cardanol and a cardol) with potential anti-cancer bioactivity. Both could be alternative antiproliferative agents for future development as anti-cancer drugs.

【 授权许可】

Unknown   
© Teerasripreecha et al; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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