A proteomics study of the mung bean epicotyl regulated by brassinosteroids under conditions of chilling stress

Cellular & Molecular Biology Letters

Bin Huang⁴  Hsueh-Fen Juan²  Chien-Hua Chu⁴  Yih-Ming Chen¹  Shu-Ling Chen³ 

[1] Institute of Plant Biology, National Taiwan University, Taipei, Taiwan$$Department of Life Science, National Taiwan University, Taipei, Taiwan$$;Department of Life Science, National Taiwan University, Taipei, Taiwan$$Institute of Molecular and Cellular Biology, National Taiwan University, Taipei, Taiwan$$;Department of Biotechnology, Ming Chuan University, Taoyuan, Taiwan$$;Institute of Plant Biology, National Taiwan University, Taipei, Taiwan$$

关键词: Proteomics;    Chilling;    Brassinosteroid;    Mung bean;    Epicotyl;   

DOI  :  10.2478/s11658-006-0021-7

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

Mung bean CYP90A2 is a putative brassinosteroid (BR) synthetic gene that shares 77% identity with the Arabidopsis CPD gene. It was strongly suppressed by chilling stress. This implies that exogenous treatment with BR could allow the plant to recover from the inhibited growth caused by chilling. In this study, we used proteomics to investigate whether the mung bean epicotyl can be regulated by brassinosteroids under conditions of chilling stress. Mung bean epicotyls whose growth was initially suppressed by chilling partly recovered their ability to elongate after treatment with 24-epibrassinolde; 17 proteins down-regulated by this chilling were re-up-regulated. These up-regulated proteins are involved in methionine assimilation, ATP synthesis, cell wall construction and the stress response. This is consistent with the re-up-regulation of methionine synthase and S-adenosyl-L-methionine synthetase, since chilling-inhibited mung bean epicotyl elongation could be partially recovered by exogenous treatment with DL-methionine. This is the first proteome established for the mung bean species. The regulatory relationship between brassinosteroids and chilling conditions was investigated, and possible mechanisms are discussed herein.

【 授权许可】

Unknown