Effect of macromolecular crowding on the rate of diffusion-limited enzymatic reaction

Pramana

Rajat Anand²  S B Santra2¹  Manish Agrawal²  Rajaram Swaminathan² 

[1] Department of Physics, Indian Institute of Technology Guwahati, Guwahati 781 039, India$$;Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781 039, India$$

关键词: Enzyme kinetics;    Monte Carlo;    percolation;    random walk;    obstacle.;   

DOI  :  

学科分类：物理（综合）

来源: Indian Academy of Sciences

【 摘 要 】

The cytoplasm of a living cell is crowded with several macromolecules of different shapes and sizes. Molecular diffusion in such a medium becomes anomalous due to the presence of macromolecules and diffusivity is expected to decrease with increase in macromolecular crowding. Moreover, many cellular processes are dependent on molecular diffusion in the cell cytosol. The enzymatic reaction rate has been shown to be affected by the presence of such macromolecules. A simple numerical model is proposed here based on percolation and diffusion in disordered systems to study the effect of macromolecular crowding on the enzymatic reaction rates. The model qualitatively explains some of the experimental observations.

【 授权许可】

Unknown