Covalent immobilization of myosin for in-vitro motility of actin

Pramana

Ellis Bagga¹  Rakesh Kumar¹  Lalit M Bharadwaj1¹  Sunita Kumari¹  R P Bajpai¹  Rajesh Kumar¹ 

[1] Biomolecular Electronics and Nanotechnology Division, Central Scientific Instrument Organisation, Sector 30/C, Chandigarh 160 030, India$$

关键词: Molecular motors;    atomic force microscope (AFM);    immobilization;    biomolecular electronics and nanotechnology.;   

DOI  :  

学科分类：物理（综合）

来源: Indian Academy of Sciences

【 摘 要 】

The present study reports the covalent immobilization of myosin on glass surface and in-vitro motility of actin-myosin biomolecular motor. Myosin was immobilized on poly-L-lysine coated glass using heterobifunctional cross linker EDC and characterized by AFM. The in-vitro motility of actin was carried out on the immobilized myosin. It was observed that velocity of actin over myosin increases with increasing actin concentration (0.4-1.0 mg/ml) and was found in the range of 0.40-3.25 𝜇m/s. The motility of actin-myosin motor on artificial surfaces is of immense importance for developing nanodevices for healthcare and engineering applications.

【 授权许可】

Unknown