Pramana | |
Covalent immobilization of myosin for in-vitro motility of actin | |
Ellis Bagga1  Rakesh Kumar1  Lalit M Bharadwaj11  Sunita Kumari1  R P Bajpai1  Rajesh Kumar1  | |
[1] Biomolecular Electronics and Nanotechnology Division, Central Scientific Instrument Organisation, Sector 30/C, Chandigarh 160 030, India$$ | |
关键词: Molecular motors; atomic force microscope (AFM); immobilization; biomolecular electronics and nanotechnology.; | |
DOI : | |
学科分类:物理(综合) | |
来源: Indian Academy of Sciences | |
【 摘 要 】
The present study reports the covalent immobilization of myosin on glass surface and in-vitro motility of actin-myosin biomolecular motor. Myosin was immobilized on poly-L-lysine coated glass using heterobifunctional cross linker EDC and characterized by AFM. The in-vitro motility of actin was carried out on the immobilized myosin. It was observed that velocity of actin over myosin increases with increasing actin concentration (0.4-1.0 mg/ml) and was found in the range of 0.40-3.25 ðœ‡m/s. The motility of actin-myosin motor on artificial surfaces is of immense importance for developing nanodevices for healthcare and engineering applications.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912040496963ZK.pdf | 290KB | download |